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Invitrogen
SMAD6 Polyclonal Antibody
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FIGURE: 1 / 6
SMAD6 Antibody (PA1-41026) in ICC/IF
Immunocytochemistry analysis of SMAD6 in HepG2 cells. Samples were incubated with SMAD6 polyclonal antibody (Product # PA1-41026) using a dilution of 1:100 dilution overnight at 4°C followed by anti-rabbit DyLight 488 (Green) at a dilution of 1:500. Cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. Alpha tubulin was used as a co-stain at a 1:1,000 dilution and detected with an anti-mouse DyLight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue)... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA1-41026
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Non-human primate,
Sheep,
Rat
Published species
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide made to an internal portion of human SMAD6 (between amino acids 30-150).
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody is predicted to react with equine based on sequence homology.
Suggested positive control: HeLa or NIH-3T3 whole cell lysate.
Cross reacts with New World Monkey.
Target Information
SMAD6 (mothers against decapentaplegic homolog 6) is an antagonist of signaling by TGF-beta (transforming growth factor) type 1 receptor superfamily members; has been shown to inhibit selectively BMP (bone morphogenetic proteins) signaling by competing with the co-SMAD SMAD4 for receptor-activated SMAD1. SMAD6 is an inhibitory SMAD (I-SMAD) or antagonistic SMAD.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: MAD homolog 6; mad homolog 7; mothers against decapentaplegic homolog 6; mothers against decapentaplegic, drosophila, homolog of, 6; mothers against DPP homolog 6; SMAD; Smad 6; SMAD family member; SMAD SMAD; SMAD, mothers against DPP homolog 6; unnamed protein product
Gene Aliases: AOVD2; b2b390Clo; HsT17432; MADH6; MADH7
UniProt ID: (Human) O43541, (Mouse) O35182
Entrez Gene ID: (Sheep) 101103068, (Human) 4091, (Mouse) 17130, (Rat) 367100
transcription regulatory region sequence-specific DNA binding
chromatin binding
protein binding
ubiquitin protein ligase binding
type I transforming growth factor beta receptor binding
identical protein binding
co-SMAD binding
I-SMAD binding
R-SMAD binding
activin receptor binding
type I activin receptor binding
protein sequestering activity
transcription regulator inhibitor activity
protein binding, bridging involved in substrate recognition for ubiquitination
DNA-binding transcription factor
gene-specific transcriptional regulator
ureteric bud development
outflow tract septum morphogenesis
aortic valve morphogenesis
mitral valve morphogenesis
pulmonary valve morphogenesis
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
immune response
zygotic specification of dorsal/ventral axis
negative regulation of cell proliferation
anatomical structure morphogenesis
cell differentiation
negative regulation of ossification
BMP signaling pathway
negative regulation of transforming growth factor beta receptor signaling pathway
negative regulation of BMP signaling pathway
cell-substrate adhesion
response to lipopolysaccharide
negative regulation of activin receptor signaling pathway
negative regulation of MyD88-dependent toll-like receptor signaling pathway
response to laminar fluid shear stress
negative regulation of apoptotic process
proteasome-mediated ubiquitin-dependent protein catabolic process
response to estrogen
fat cell differentiation
negative regulation of cell differentiation
negative regulation of osteoblast differentiation
negative regulation of SMAD protein import into nucleus
SMAD protein signal transduction
cellular response to growth factor stimulus
transforming growth factor beta receptor superfamily signaling pathway
positive regulation of pri-miRNA transcription from RNA polymerase II promoter
heart valve development
ventricular septum development
aorta development
vasoconstriction
positive regulation of vasoconstriction
cardiac vascular smooth muscle cell development
coronary vasculature development
coronary vasculature morphogenesis
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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