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Invitrogen
DHFR Polyclonal Antibody
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Product Details
PA5-30992
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rat
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human DHFR. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
1.54 mg/mL
Amount
154 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Recommended positive controls: 293T, A431, PC-12.
Predicted reactivity: Mouse (88%), Rat (89%), Pig (88%), Bovine (85%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Dihydrofolate reductase converts dihydrofolate into tetrahydrofolate, and is required for the synthesis of purines, thymidylic acid and certain amino acids. While the functional dihydrofolate reductase gene has been mapped to chromosome 5, multiple intronless processed pseudogenes or dihydrofolate reductase like genes have been identified on separate chromosomes. Dihydrofolate reductase deficiency has been linked to megaloblastic anemia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Dihydrofolate reductase; Dihydrofolate reductase 1 (active); unnamed protein product
Gene Aliases: DHFR; DHFR1; DHFRP1; DYR
UniProt ID: (Human) P00374, (Rat) Q920D2
Entrez Gene ID: (Human) 1719, (Rat) 24312
tetrahydrobiopterin biosynthetic process
one-carbon metabolic process
negative regulation of translation
axon regeneration
response to methotrexate
dihydrofolate metabolic process
tetrahydrofolate metabolic process
tetrahydrofolate biosynthetic process
folic acid metabolic process
regulation of removal of superoxide radicals
glycine biosynthetic process
nucleotide biosynthetic process
response to nicotine
oxidation-reduction process
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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