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Invitrogen
Nhe-1 Polyclonal Antibody
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Product Details
PA5-62703
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein corresponding to Human Nhe-1. Recombinant protein control fragment (Product #RP-103140).
Conjugate
Form
Liquid
Concentration
0.1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 40% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: SPESVDLVNE ELKGKVLGLS RDPAKVAEED EDDDGGIMMR SKETSSPGTD DVFTPAPSDS PSSQRIQRCL SDPGPHPEPG EGEPFFPKG
Highest antigen sequence identity to the following orthologs: Mouse - 83%, Rat - 83%.
HIER pH 6 was used for IHC antigen retrieval. Cells used for ICC/IF were permeabilized and fixed with PFA/Triton™ X-100.
Target Information
The Na+/H+ antiporter (Nhe-1) is a ubiquitous membrane-bound enzyme involved in pH regulation of vertebrate cells and is specifically inhibited by the diuretic drug amiloride and activated by a variety of signals including growth factors, mitogens, neurotransmitters, and tumor promoters. Nhe-1 acts as an anchor for actin filaments to control the integrity of the cortical cytoskeleton. This occurs through a previously unrecognized structural link between Nhe-1 and the actin-binding proteins ezrin, radixin, and moesin, collectively referred to as ERM proteins. A structural role for Nhe-1 has been proposed in regulating the cortical cytoskeleton that is independent of its function as an ion exchanger. It is also thought that Nhe-1 play a role in hypertension. At least two isoforms of Nhe-1 are known to exist.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: APNH; Na(+)/H(+) antiporter, amiloride-sensitive; Na(+)/H(+) exchanger 1; Na-Li countertransporter; NHE-1; protein phosphatase 1, regulatory subunit 143; Sodium/hydrogen exchanger 1; solute carrier family 9 (sodium/hydrogen exchanger), isoform 1 (antiporter, Na+/H+, amiloride sensitive); solute carrier family 9 (sodium/hydrogen exchanger), member 1 (antiporter, Na+/H+, amiloride sensitive); Solute carrier family 9 member 1; solute carrier family 9, subfamily A (NHE1, cation proton antiporter 1), member 1
Gene Aliases: APNH; APNH1; LIKNS; NHE-1; NHE1; PPP1R143; SLC9A1
UniProt ID: (Human) P19634
Entrez Gene ID: (Human) 6548
protein binding
calmodulin binding
phosphatidylinositol-4,5-bisphosphate binding
solute:proton antiporter activity
sodium:proton antiporter activity
potassium:proton antiporter activity
protein phosphatase 2B binding
protein binding, bridging
protein complex scaffold
calcium-dependent protein binding
sodium:proton antiporter activity involved in regulation of cardiac muscle cell membrane potential
transporter
secondary carrier transporter
ion transport
cellular sodium ion homeostasis
regulation of pH
response to acidic pH
positive regulation of cardiac muscle hypertrophy
regulation of cardiac muscle contraction by calcium ion signaling
cell growth
cell migration
maintenance of cell polarity
hyaluronan catabolic process
positive regulation of cell growth
cellular response to insulin stimulus
positive regulation of mitochondrial membrane permeability
response to muscle stretch
response to drug
positive regulation of apoptotic process
negative regulation of apoptotic process
positive regulation of action potential
positive regulation of transcription from RNA polymerase II promoter
protein oligomerization
regulation of intracellular pH
regulation of stress fiber assembly
positive regulation of NFAT protein import into nucleus
regulation of focal adhesion assembly
regulation of sensory perception of pain
cardiac muscle cell differentiation
positive regulation of calcineurin-NFAT signaling cascade
neuron death
cellular response to antibiotic
cellular response to electrical stimulus
cellular response to mechanical stimulus
sodium ion export
cellular response to hypoxia
cellular response to acidic pH
potassium ion transmembrane transport
cellular response to epinephrine stimulus
cardiac muscle cell contraction
regulation of cardiac muscle cell membrane potential
regulation of the force of heart contraction by cardiac conduction
sodium ion import across plasma membrane
positive regulation of the force of heart contraction
hydrogen ion transmembrane transport
positive regulation of calcium:sodium antiporter activity
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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