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Invitrogen
SIRP alpha Polyclonal Antibody
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SIRP alpha Antibody (PA5-84384) in IHC (P)
Immunohistochemistry analysis of SIRP alpha in human cerebellum, cerebral cortex, pancreas and skeletal muscle. Samples were incubated with SIRP alpha polyclonal antibody (Product # PA5-84384). Shows similar protein distribution across tissues to independent SIRP alpha antibody (B).
Product Details
PA5-84384
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein corresponding to Human SIRPA. Recombinant protein control fragment (Product #RP-105105).
Conjugate
Form
Liquid
Concentration
0.05 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 40% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: RIRQKKAQGS TSSTRLHEPE KNAREITQDT NDITYADLNL PKGKKPAPQA AEPN
Highest antigen sequence indentity to the following orthologs: Mouse - 81%, Rat - 80%.
Target Information
SIRP alpha (CD172a, signal-regulatory protein alpha) is a receptor-type transmembrane glycoprotein expressed on cells of myeloid origin, including granulocytes, dendritic cells (DCs), macrophages, mast cells and hematopoietic stem cells. SIRP alpha acts as a substrate for several activated tyrosine kinases, including EGFR, PDGFR, src and insulin receptor and is involved in the negative regulation of receptor tyrosine kinase-coupled signaling pathways. The ligand binding of SIRP alpha to integrin-associated protein CD47 results in tyrosine kinase phosphorylation of immunoreceptor tyrosine-based inhibitory motifs (ITIMs) within the cytoplasmic region of SIRP alpha, which mediates the recruitment and activation of the tyrosine phosphatases SHP-1 and SHP-2. Ligation of SIRP alpha with CD47 has been demonstrated in several regulatory processes, including the inhibition of host cell phagocytosis by macrophages and the bi-directional activation of T cells and DCs. SIRP alpha has regulatory effects on cellular responses induced by serum, growth factors, insulin, oncogenes, growth hormones and cell adhesion, and plays a general role in different physiological and pathological processes. Cancer cells highly express CD47, which activates SIRP alpha and inhibits macrophage-mediated destruction of cancerous cell growth.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Bit; Brain Ig-like molecule with tyrosine-based activation motifs; brain-immunoglobulin-like molecule with tyrosine-based activation motifs; CD172 alpha; CD172 antigen-like family member A; CD172a; Inhibitory receptor SHPS-1; Macrophage fusion receptor; MyD-1 antigen; p84; SHP substrate 1; signal-regulatory protein alpha; Signal-regulatory protein alpha-1; Signal-regulatory protein alpha-2; Signal-regulatory protein alpha-3; Sirp-alpha-1; Sirp-alpha-2; Sirp-alpha-3; SIRPalpha; SIRPalpha2; tyrosine phosphatase SHP substrate 1; Tyrosine-protein phosphatase non-receptor type substrate 1
Gene Aliases: BIT; CD172A; MFR; MYD-1; MYD1; P84; PTPNS1; SHPS1; SIRP; SIRPA
UniProt ID: (Human) P78324
Entrez Gene ID: (Human) 140885
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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