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Invitrogen
Phospho-CDC25C (Ser216) Polyclonal Antibody
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FIGURE: 1 / 3
Phospho-CDC25C (Ser216) Antibody (PA5-104244) in ICC/IF
Immunofluorescent analysis of Phospho-CDC25C (Ser216) in HeLa cells(4h of LPS treatment). Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and Phospho-CDC25C (Ser216) polyclonal antibody (Product # PA5-104244) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor ... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-104244
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human CDC25C(Accession P30307), corresponding to amino acid residues around phosphorylated Ser216.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
sequential chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of CDC25C only when phosphorylated at Serine 216.
Target Information
M-phase inducer phosphatase 3is an enzyme that in humans is encoded by theCDC25Cgene. This gene is highly conserved during evolution and it plays a key role in the regulation of cell division. The encoded protein is a tyrosine phosphatase and belongs to the Cdc25 phosphatase family. It directs dephosphorylation of cyclin B-bound CDC2 (CDK1) and triggers entry into mitosis. Also, it is thought to suppress p53-induced growth arrest. Multiple alternatively spliced transcript variants of this gene have been described, however, the full-length nature of many of them is not known.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CDC25 homolog C; CDC25C2; CDC25C3; CDC25C4; CDC25C5; CDC25Hs ORF; cell cycle phosphatase CDC25C; cell division cycle control protein 25C; dual specificity phosphatase CDC25C; EC 3.1.3.48; mitosis inducer CDC25; MPIP3; OTTHUMP00000159490; OTTHUMP00000224012; OTTHUMP00000224013; OTTHUMP00000224016; phosphotyrosine phosphatase; protein phosphatase 1, regulatory subunit 60; unnamed protein product
Gene Aliases: CDC25; PPP1R60
UniProt ID: (Human) P30307, (Mouse) P48967
Entrez Gene ID: (Human) 995, (Mouse) 12532
regulation of cyclin-dependent protein serine/threonine kinase activity
G2/M transition of mitotic cell cycle
regulation of mitotic nuclear division
cell proliferation
positive regulation of G2/M transition of mitotic cell cycle
positive regulation of G2/MI transition of meiotic cell cycle
positive regulation of cell cycle G2/M phase transition
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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