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Invitrogen
LIGHT Polyclonal Antibody
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FIGURE: 1 / 6
LIGHT Antibody (PA5-104479) in ICC/IF
Immunofluorescent analysis of LIGHT in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and LIGHT polyclonal antibody (Product # PA5-104479) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-104479
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human TNFSF14(Accession O43557), corresponding to amino acid residues L61-P111.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total TNFSF14.
Target Information
Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Apoptosis is induced by certain cytokines including TNF (tumor necrosis factor) and Fas ligand of the TNF family through their death domain containing receptors, TNFR1 (tumor necrosis factor receptor 1) and Fas. Cell death signals are transduced by death domain (DD)-containing adapter molecules and members of the ICE (interleukin-1-beta converting enzyme)/CED-3 (C. elegans death receptor-3) protease family. A novel DD-containing molecule was recently cloned from mouse, human and monkey and designated Daxx (death associated protein 6). Daxx binds specifically to the Fas death domain and enhances Fas induced apoptosis and activates the Jun N-terminal kinase (JNK) pathway. Daxx is widely expressed in fetal and adult human and mouse tissues indicating its important function in Fas signaling pathways.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: herpesvirus entry mediator ligand; HVEM-L; TNF homolog; LIGHT; sCD258; sLIGHT; soluble CD258; soluble LIGHT; TNFSF14; TR2; tumor necrosis factor (ligand) superfamily, member 14; tumor necrosis factor ligand 1D; tumor necrosis factor superfamily member 14; type II transmembrane protein; unnamed protein product
Gene Aliases: CD258; HVEM-L; HVEML; LIGHT; LTg; Ly113; Tnlg1d
UniProt ID: (Human) O43557, (Mouse) Q9QYH9
Entrez Gene ID: (Human) 8740, (Mouse) 50930
immune response
cell surface receptor signaling pathway
positive regulation of T cell chemotaxis
T cell costimulation
positive regulation of I-kappaB kinase/NF-kappaB signaling
positive regulation of myoblast differentiation
cellular response to mechanical stimulus
positive regulation of NIK/NF-kappaB signaling
positive regulation of myoblast fusion
positive regulation of extrinsic apoptotic signaling pathway
apoptotic process
cell communication
signal transduction
signaling
regulation of interferon-gamma production
T cell proliferation
T cell activation
T cell homeostasis
negative regulation of apoptotic process
negative regulation of inflammatory response
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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