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Invitrogen
Phospho-Tyrosine Hydroxylase (Ser19) Polyclonal Antibody
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FIGURE: 1 / 5
Phospho-Tyrosine Hydroxylase (Ser19) Antibody (PA5-104765) in ICC/IF
Immunofluorescent analysis of Phospho-Tyrosine Hydroxylase (Ser19) in HeLa cells(4h of LPS treatment). Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and Phospho-Tyrosine Hydroxylase (Ser19) polyclonal antibody (Product # PA5-104765) using a dilution of 1:200 (1 hr, 37°C), and followed by goat an... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-104765
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human TH(Accession P07101), corresponding to amino acid residues around phosphorylated Ser19.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
sequential chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of Tyrosine Hydroxylase only when phosphorylated at Serine 19.
Target Information
Tyrosine hydroxylase (TH) is an enzyme involved in the synthesis of catecholamine neurotransmitters dopamine, epinephrine, and norepinephrine. In all species, catecholamine synthesis is regulated by the interaction of TH with a cofactor, tetrahydrobiopterin (BH4). BH4 binds to the TH catalytic domain, resulting in enzymatic activity. Unlike TH in non-primate species, four human TH mRNA splice variants (hTH1-hTH4) have been isolated. These variants are identical in their catalytic domain, but differ in their N-terminal, regulatory domains. TH is also responsible for the conversion of L-tyrosine to L-dopa. TH plays a key role in the physiology of adrenergic neurons. The role of TH in the synthesis of catecholamine neurotransmitters suggests a correlation between the enzyme and a number of neuropathogenic diseases including: Parkinson's disease, schizophrenia, Segawa syndrome, and dystonia, as well as a variety of cardiovascular diseases.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: aromatic amino acid hydroxylase; dystonia 14; EC 1.14.16.2; HGNC:11782; TH isoform 3; TH isoform a; TH-4; TY3H; TYH antibody; tyrosine 3-hydroxylase; tyrosine hydroxylase (EC 1.14.16.2); tyrosine hydroxylase type 4 (EC 1.14.16.2); unnamed protein product
Gene Aliases: DYT14; DYT5b; The; TYH
UniProt ID: (Human) P07101, (Mouse) P24529, (Rat) P04177
Entrez Gene ID: (Human) 7054, (Mouse) 21823, (Rat) 25085
monooxygenase activity
tyrosine 3-monooxygenase activity
iron ion binding
protein binding
oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced pteridine as one donor, and incorporation of one atom of oxygen
enzyme binding
identical protein binding
ferrous iron binding
ferric iron binding
amino acid binding
oxygen binding
protein domain specific binding
tetrahydrobiopterin binding
dopamine binding
small molecule binding
response to hypoxia
synaptic transmission, dopaminergic
dopamine biosynthetic process from tyrosine
heart development
visual perception
learning
memory
mating behavior
locomotory behavior
regulation of heart contraction
aromatic amino acid family metabolic process
organ morphogenesis
aminergic neurotransmitter loading into synaptic vesicle
dopamine biosynthetic process
epinephrine biosynthetic process
norepinephrine biosynthetic process
catecholamine biosynthetic process
eye photoreceptor cell development
eating behavior
response to leptin
response to ethanol
embryonic camera-type eye morphogenesis
cognition
hyaloid vascular plexus regression
heart morphogenesis
anatomical structure morphogenesis
pigmentation
response to amphetamine
fatty acid metabolic process
sphingolipid metabolic process
response to xenobiotic stimulus
response to light stimulus
response to herbicide
response to salt stress
response to metal ion
response to zinc ion
response to activity
response to insecticide
phthalate metabolic process
cerebral cortex development
response to nutrient levels
response to estradiol
response to lipopolysaccharide
response to nicotine
social behavior
response to isolation stress
response to immobilization stress
circadian sleep/wake cycle
response to peptide hormone
response to ether
response to pyrethroid
response to steroid hormone
response to corticosterone
response to electrical stimulus
response to growth factor
cellular response to manganese ion
cellular response to alkaloid
cellular response to nicotine
cellular response to glucose stimulus
cellular response to growth factor stimulus
cellular response to xenobiotic stimulus
response to curcumin
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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