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Invitrogen

CASPASE 1 P10 (CLEAVED ALA317) Polyclonal Antibody

2 Published Figures
5 References

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Product Details

PA5-105049

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200
View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:100-1:500
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human CASP1(Accession P29466), corresponding to amino acid residues T310-S332.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Amount

100 µg

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2816522

Product Specific Information

Antibody detects endogenous levels of fragment of activated Caspase-1 resulting from cleavage adjacent to Ala317.

Target Information

Caspase-1 p10 (cleaved Ala317) refers to the small, active subunit generated following proteolytic processing of pro-caspase-1, an inflammatory cysteine protease encoded by the CASP1 gene. Caspase-1 is synthesized as an inactive zymogen that becomes activated within multiprotein inflammasome complexes in response to pathogenic infection or cellular stress. Activation involves autocatalytic cleavage at specific aspartic acid residues, including near alanine 317, resulting in the formation of the p20 (large) and p10 (small) subunits that assemble into the catalytically active enzyme. The cleaved p10 fragment is therefore a marker of caspase-1 activation. Active caspase-1 mediates the maturation and secretion of pro-inflammatory cytokines such as interleukin-1β (IL-1β) and interleukin-18 (IL-18), and plays a central role in pyroptosis, a form of inflammatory programmed cell death. Detection of cleaved caspase-1 p10 (Ala317) is widely used as an indicator of inflammasome activation in immunology and inflammation research.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Active Caspase-1 (p10); CASP-1; caspase 1, apoptosis-related cysteine peptidase; Caspase-1; Caspase-1 (cleaved); Cleaved Caspase-1; cysteine protease; ICE; IL-1 beta-converting enzyme; IL-1B converting enzyme; IL-1BC; IL1B-convertase; interleukin 1 beta-converting enzyme; interleukin 1, beta, convertase; interleukin 1-B converting enzyme; Interleukin 1beta converting enzyme; Interleukin-1 beta convertase; Interleukin-1 beta-converting enzyme; p45; unnamed protein product

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Gene Aliases: CASP1; ICE; IL1BC; IL1BCE; P45

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UniProt ID: (Human) P29466, (Mouse) P29452

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Entrez Gene ID: (Human) 834, (Mouse) 12362, (Rat) 25166

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Function(s)
endopeptidase activity cysteine-type endopeptidase activity protein binding peptidase activity cysteine-type peptidase activity cysteine-type endopeptidase activator activity involved in apoptotic process hydrolase activity enzyme binding kinase binding cytokine binding identical protein binding CARD domain binding scaffold protein binding protease
Process(es)
negative regulation of transcription from RNA polymerase II promoter plasma membrane repair pattern recognition receptor signaling pathway proteolysis apoptotic process inflammatory response signal transduction osmosensory signaling pathway protein secretion protein autoprocessing protein catabolic process response to lipopolysaccharide positive regulation of interleukin-1 beta production positive regulation of interleukin-18 production interleukin-18-mediated signaling pathway defense response to bacterium regulation of apoptotic process positive regulation of I-kappaB kinase/NF-kappaB signaling icosanoid biosynthetic process ceramide biosynthetic process regulation of inflammatory response positive regulation of inflammatory response protein maturation defense response to virus protein poly-ADP-ribosylation pyroptosis interleukin-1-mediated signaling pathway cellular response to lipopolysaccharide cellular response to mechanical stimulus cellular response to interferon-gamma cytokine precursor processing signaling receptor ligand precursor processing AIM2 inflammasome complex assembly pyroptotic cell death positive regulation of tumor necrosis factor-mediated signaling pathway response to hypoxia microglial cell activation myoblast fusion memory response to bacterium regulation of autophagy response to organic cyclic compound protein processing interleukin-1 beta production response to ATP response to drug positive regulation of apoptotic process positive regulation of circadian sleep/wake cycle, non-REM sleep positive regulation of cytokine secretion positive regulation of interleukin-1 alpha secretion positive regulation of interleukin-1 beta secretion mitochondrial depolarization membrane hyperpolarization cellular response to organic substance programmed necrotic cell death toxin transport midgut development response to toxic substance lung development execution phase of apoptosis
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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