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Invitrogen
Phospho-YBX1 (Tyr162) Polyclonal Antibody
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FIGURE: 1 / 4
Phospho-YBX1 (Tyr162) Antibody (PA5-105601) in IHC (P)
Immunohistochemistry analysis of paraffin-embedded Phospho-YBX1 (Tyr162) in mouse spleen tissue sections. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with Phospho-YBX1 (Tyr162) polyclonal antibody (Product # PA5-105601) using a dilution of 1:100 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.
Product Details
PA5-105601
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human YBX1(Accession P67809), corresponding to amino acid residues around phosphorylated Tyr162.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
sequential chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of YB1 only when phosphorylated at Tyr162.
Target Information
YBX1 binds to splice sites in pre-mRNA and regulates splice site selection. This protein binds and stabilizes cytoplasmic mRNA and contributes to the regulation of translation by modulating the interaction between the mRNA and eukaryotic initiation factors. It binds to promoters that contain a Y-box (5'-CTGATTGGCCAA-3'), such as HLA class II genes. It regulates the transcription of numerous genes and promotes separation of DNA strands that contain mismatches or are modified by cisplatin. It has endonucleolytic activity and can introduce nicks or breaks into double-stranded DNA (in vitro), and it may play a role in DNA repair.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: BP8; CBF-A; CCAAT-binding; CCAAT-binding transcription factor 1 subunit A; CCAAT-binding transcription factor I subunit A; DBPB; DNA binding protein B; DNA-binding protein B; EFI-A; enhancer factor 1 alpha; enhancer factor 1 subunit A; Enhancer factor I subunit A; major histocompatibility complex, class II, Y box-binding protein I; MDR NF1; MGC104858; MGC110976; MGC117250; nuclease sensitive element binding protein 1; Nuclease-sensitive element-binding protein 1; similar to dbpB protein; Y box binding protein-1; Y box transcription factor; Y-box binding protein 1; Y-box transcription factor; Y-box-binding protein 1; YB-1
Gene Aliases: 1700102N10Rik; BP-8; Byb1; C79409; CBF-A; Cbfa; CSDA2; CSDB; DBPB; EF1A; EFI-A; MDR-NF1; Msy-1; MSY1; mYB-1a; NSEP-1; NSEP1; YB-1; YB1; YBX1
UniProt ID: (Human) P67809, (Mouse) P62960, (Rat) P62961
Entrez Gene ID: (Human) 4904, (Mouse) 22608, (Rat) 500538
nucleic acid binding
DNA binding
chromatin binding
double-stranded DNA binding
single-stranded DNA binding
RNA binding
mRNA binding
protein binding
miRNA binding
GTPase binding
C5-methylcytidine-containing RNA reader activity
sequence-specific double-stranded DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
p53 binding
transcription factor binding
sequence-specific DNA binding
poly(A) RNA binding
negative regulation of transcription from RNA polymerase II promoter
in utero embryonic development
regulation of transcription, DNA-templated
mRNA processing
RNA splicing
epidermis development
regulation of gene expression
negative regulation of translation
positive regulation of transcription from RNA polymerase II promoter
mRNA stabilization
embryonic morphogenesis
RNA transport
tRNA transport
negative regulation of striated muscle cell differentiation
positive regulation of cell division
CRD-mediated mRNA stabilization
cellular response to interleukin-7
negative regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay
protein localization to cytoplasmic stress granule
miRNA transport
positive regulation of cytoplasmic translation
negative regulation of cellular senescence
transcription, DNA-templated
transcription from RNA polymerase II promoter
positive regulation of cell proliferation
negative regulation of apoptotic process
negative regulation of insulin receptor signaling pathway
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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