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Invitrogen
HCK Polyclonal Antibody
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FIGURE: 1 / 3
HCK Antibody (PA5-105729) in ICC/IF
Immunocytochemistry analysis of HCK in HepG2 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with HCK polyclonal antibody (Product # PA5-105729) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Product Details
PA5-105729
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human HCK(Accession P08631), corresponding to amino acid residues N496-P526.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total Hck.
Target Information
Hematopoietic cell kinase (HCK) is a SRC-related kinase expressed in lymphoid and hematopoietic cells and may play a role in some forms of leukemia. (HCK Alias: DKFZp686D0638). HCK is a protein-tyrosine kinase that is predominantly expressed in hemopoietic cell types. The encoded protein may help couple the Fc receptor to the activation of the respiratory burst. In addition, it may play a role in neutrophil migration and in the degranulation of neutrophils. Alternate translation initiation site usage, including a non-AUG (CUG) codon, results in the production of two different isoforms, that have different subcellular localization.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: B cell/myeloid kinase; B-cell/myeloid kinase; BMK; Hematopoietic cell kinase; Hemopoietic cell kinase; p56-HCK/p59-HCK; p59-HCK/p60-HCK; p59Hck; p61Hck; protein-tyrosine kinase; Tyrosine-protein kinase HCK; unnamed protein product
Gene Aliases: AI849071; AIPCV; Bmk; HCK; JTK9; p59Hck; p61Hck
UniProt ID: (Human) P08631, (Mouse) P08103
Entrez Gene ID: (Human) 3055, (Mouse) 15162
nucleotide binding
phosphotyrosine binding
protein kinase activity
protein tyrosine kinase activity
non-membrane spanning protein tyrosine kinase activity
receptor binding
protein binding
ATP binding
lipid binding
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
immune system process
leukocyte migration involved in immune response
innate immune response-activating signal transduction
negative regulation of inflammatory response to antigenic stimulus
protein phosphorylation
exocytosis
phagocytosis
response to stress
inflammatory response
cell adhesion
transmembrane receptor protein tyrosine kinase signaling pathway
integrin-mediated signaling pathway
mesoderm development
positive regulation of cell proliferation
regulation of cell shape
peptidyl-tyrosine phosphorylation
cytokine-mediated signaling pathway
cell differentiation
positive regulation of actin filament polymerization
lipopolysaccharide-mediated signaling pathway
regulation of actin cytoskeleton organization
intracellular signal transduction
Fc-gamma receptor signaling pathway involved in phagocytosis
negative regulation of apoptotic process
leukocyte degranulation
innate immune response
respiratory burst after phagocytosis
protein autophosphorylation
regulation of inflammatory response
regulation of phagocytosis
defense response to Gram-positive bacterium
leukocyte migration
regulation of sequence-specific DNA binding transcription factor activity
interferon-gamma-mediated signaling pathway
cellular response to lipid
regulation of podosome assembly
phosphorylation
peptidyl-tyrosine autophosphorylation
regulation of cell proliferation
cellular response to peptide hormone stimulus
positive regulation of actin cytoskeleton reorganization
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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