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Invitrogen
Phospho-BRK (Tyr342) Polyclonal Antibody
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FIGURE: 1 / 3
Phospho-BRK (Tyr342) Antibody (PA5-105867) in IHC (P)
Immunohistochemistry analysis of paraffin-embedded Phospho-BRK (Tyr342) in mouse kidney tissue sections. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with Phospho-BRK (Tyr342) polyclonal antibody (Product # PA5-105867) using a dilution of 1:200 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-105867
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human PTK6(Accession Q13882), corresponding to amino acid residues around phosphorylated Tyr342.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
sequential chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of BRK only when phosphorylated at Tyr342.
Target Information
Breast tumor kinase (Brk) is a non-receptor protein tyrosine kinase expressed in over 60% of breast carcinoma tissue samples and breast tumor cell lines. Brk is 80% identity to Sik (Src-related intestinal kinase) and exhibits the features of a novel non-receptor tyrosine kinase, including amino terminal SH3 and SH2 domains. Low levels of Brk transcripts have been identified in some human breast tumor cell lines but not in normal breast tissue.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ALT-PTK6; breast tumor kinase; deltam5; FLJ42088; protein-tyrosine kinase BRK; PTK6 protein tyrosine kinase 6; src-related intestinal kinase; tyrosine kinase identified from skin; tyrosine-protein kinase BRK; unnamed protein product
Gene Aliases: BRK; Sik; tks; Tksk
UniProt ID: (Human) Q13882, (Mouse) Q64434
Entrez Gene ID: (Human) 5753, (Mouse) 20459, (Rat) 366275
transmembrane receptor protein tyrosine kinase signaling pathway
tyrosine phosphorylation of STAT protein
positive regulation of neuron projection development
cell migration
cell differentiation
negative regulation of growth
protein autophosphorylation
intestinal epithelial cell differentiation
negative regulation of protein tyrosine kinase activity
cellular response to retinoic acid
protein phosphorylation
ERBB2 signaling pathway
positive regulation of DNA replication
positive regulation of epidermal growth factor receptor signaling pathway
positive regulation of cell cycle
positive regulation of JAK-STAT cascade
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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