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Invitrogen
Phospho-MEK5 (Thr315) Polyclonal Antibody
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FIGURE: 1 / 3
Phospho-MEK5 (Thr315) Antibody (PA5-105899) in IHC (P)
Immunohistochemistry analysis of paraffin-embedded Phospho-MEK5 (Thr315) in mouse kidney tissue sections. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with Phospho-MEK5 (Thr315) polyclonal antibody (Product # PA5-105899) using a dilution of 1:200 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-105899
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human MAP2K5(Accession Q13163), corresponding to amino acid residues around phosphorylated Thr315.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
sequential chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of MEK5 only when phosphorylated at Thr315.
Target Information
MAP2K5 (MEK5) serine/threonine kinase (mitogen-activated protein kinase 5) or MEK5 is a dual specificity protein kinase that belongs to the MAP kinase family which specifically interacts with and activates MAPK7/ERK5. MAP2K5 itself can be phosphorylated and activated by MAP3K3/MEKK3, as well as by atypical protein kinase C isoforms (aPKCs). The signal cascade mediated by MAP2K5 is involved in growth factor stimulated cell proliferation and muscle cell differentiation. The rs3743354 polymorphism in the MAP2K5 promoter may affect the risk of developing colorectal cancer (CRC).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: MAP kinase kinase 5; MAP kinase kinase MEK5b; MAP2K5; MAPK/ERK kinase 5; MAPKK 5; MEK 5; unnamed protein product
Gene Aliases: HsT17454; MAPKK5; MEK5; PRKMK5
UniProt ID: (Human) Q13163, (Rat) Q62862, (Mouse) Q9WVS7
Entrez Gene ID: (Human) 5607, (Rat) 29568, (Mouse) 23938
negative regulation of transcription from RNA polymerase II promoter
MAPK cascade
protein phosphorylation
heart development
positive regulation of cell growth
negative regulation of interleukin-8 production
negative regulation of heterotypic cell-cell adhesion
negative regulation of smooth muscle cell apoptotic process
negative regulation of I-kappaB kinase/NF-kappaB signaling
positive regulation of transcription from RNA polymerase II promoter
insulin-like growth factor receptor signaling pathway
positive regulation of epithelial cell proliferation
positive regulation of protein metabolic process
negative regulation of response to cytokine stimulus
ERK5 cascade
cellular response to growth factor stimulus
negative regulation of cell migration involved in sprouting angiogenesis
negative regulation of chemokine (C-X-C motif) ligand 2 production
negative regulation of extrinsic apoptotic signaling pathway in absence of ligand
signal transduction
cellular response to laminar fluid shear stress
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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