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Invitrogen
AKR1C3 Polyclonal Antibody
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FIGURE: 1 / 8
AKR1C3 Antibody (PA5-106891) in ICC/IF
Immunocytochemistry analysis of AKR1C3 in A549 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with AKR1C3 polyclonal antibody (Product # PA5-106891) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-106891
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human AKR1C3(Accession P42330), corresponding to amino acid residues E291-Y323.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total AKR1C3.
Target Information
AKR1C3 a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme catalyzes the reduction of prostaglandin (PG) D2, PGH2 and phenanthrenequinone (PQ), and the oxidation of 9-alpha,11-beta-PGF2 to PGD2. It may play an important role in the pathogenesis of allergic diseases such as asthma, and may also have a role in controlling cell growth and/or differentiation. This gene shares high sequence identity with three other gene members and is clustered with those three genes at chromosome 10p15-p14.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 17beta-HSD5; 2-dih; 20-alpha-HSD; 20-alpha-hydroxysteroid dehydrogenase; 20alpha-hydroxysteroid dehydrogenase; 3-alpha hydroxysteroid dehydrogenase, type II; 3-alpha-HSD type II, brain; aldo-keto reductase family 1 member 18; aldo-keto reductase family 1, member C18; aldo-keto reductase family 1, member C3 (3-alpha hydroxysteroid dehydrogenase, type II); chlordecone reductase homolog; chlordecone reductase homolog HAKRb; dihydrodiol dehydrogenase; dihydrodiol dehydrogenase 3; dihydrodiol dehydrogenase X; HSD; HSD1; indanol dehydrogenase; KIAA0119; prostaglandin F synthase; testosterone 17-beta-dehydrogenase 5; trans-1,2-dihydrobenzene-1,2-diol dehydrogenase; type IIb 3-alpha hydroxysteroid dehydrogenase; unnamed protein product
Gene Aliases: Akr1c18; DD3; DDX; HA1753; HAKRB; HAKRe; hluPGFS; HSD17B5; PGFS
UniProt ID: (Human) P42330, (Rat) P51652
Entrez Gene ID: (Human) 8644, (Rat) 171516
retinal dehydrogenase activity
alditol:NADP+ 1-oxidoreductase activity
estradiol 17-beta-dehydrogenase activity
retinol dehydrogenase activity
protein binding
alcohol dehydrogenase (NADP+) activity
oxidoreductase activity
oxidoreductase activity, acting on NAD(P)H, quinone or similar compound as acceptor
bile acid binding
prostaglandin H2 endoperoxidase reductase activity
prostaglandin D2 11-ketoreductase activity
17-beta-hydroxysteroid dehydrogenase (NAD+) activity
ketoreductase activity
prostaglandin-F synthase activity
15-hydroxyprostaglandin-D dehydrogenase (NADP+) activity
androsterone dehydrogenase activity
5-alpha-androstane-3-beta,17-beta-diol dehydrogenase (NADP+) activity
testosterone dehydrogenase (NAD+) activity
androstan-3-alpha,17-beta-diol dehydrogenase activity
testosterone 17-beta-dehydrogenase (NADP+) activity
ketosteroid monooxygenase activity
delta4-3-oxosteroid 5beta-reductase activity
NADP-retinol dehydrogenase activity
17-beta-hydroxysteroid dehydrogenase (NADP+) activity
geranylgeranyl diphosphate reductase activity
3-alpha-hydroxysteroid 3-dehydrogenase [NAD(P)+] activity
17-alpha,20-alpha-dihydroxypregn-4-en-3-one dehydrogenase activity
prostaglandin metabolic process
progesterone catabolic process
G-protein coupled receptor signaling pathway
female pregnancy
parturition
response to nutrient
steroid metabolic process
positive regulation of cell proliferation
male gonad development
cellular response to starvation
farnesol catabolic process
keratinocyte differentiation
progesterone metabolic process
retinal metabolic process
daunorubicin metabolic process
doxorubicin metabolic process
regulation of retinoic acid receptor signaling pathway
regulation of steroid biosynthetic process
positive regulation of protein kinase B signaling
testosterone biosynthetic process
cellular response to calcium ion
cellular response to follicle-stimulating hormone stimulus
cellular response to prostaglandin stimulus
cellular response to corticosteroid stimulus
cellular response to testosterone stimulus
cellular response to jasmonic acid stimulus
cellular response to transforming growth factor beta stimulus
cellular response to prostaglandin D stimulus
cellular response to gonadotropin-releasing hormone
negative regulation of retinoic acid biosynthetic process
cellular response to forskolin
cellular response to prolactin
regulation of testosterone biosynthetic process
positive regulation of endothelial cell apoptotic process
positive regulation of reactive oxygen species metabolic process
retinoid metabolic process
monocarboxylic acid metabolic process
hormone metabolic process
retinol metabolic process
macromolecule metabolic process
prostanoid biosynthetic process
renal absorption
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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