Search
Invitrogen
EphB1 Polyclonal Antibody
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
Additional Information:
{{banner.description}}
FIGURE: 1 / 1
EphB1 Antibody (PA5-109688) in WB
Western blot analysis of EphB1 in mouse kidney. Samples were incubated with EphB1 Polyclonal antibody (Product # PA5-109688) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
Product Details
PA5-109688
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 260-500 of human EPHB1 (NP_004432.1).
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1.25 mg/mL
Amount
125 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: EPENSVACKA CPAGTFKASQ EAEGCSHCPS NSRSPAEASP ICTCRTGYYR ADFDPPEVAC TSVPSGPRNV ISIVNETSII LEWHPPRETG GRDDVTYNII CKKCRADRRS CSRCDDNVEF VPRQLGLTEC RVSISSLWAH TPYTFDIQAI NGVSSKSPFP PQHVSVNITT NQAAPSTVPI MHQVSATMRS ITLSWPQPEQ PNGIILDYEI RYYEKEHNEF NSSMARSQTN TARIDGLRPG M
Target Information
EPHB1 is a receptor tyrosine kinase that belongs to the ephrin receptor family. Members of the Eph family of kinases play important roles in diverse biological processes including nervous system development, angiogenesis and neural synapsis formation and maturation. EphB1 and other ephB family members are type 1 membrane spanning proteins, comprised of immunoglobulin, fibronectin type III, and cysteine rich subdomains in the ecto domain, and the single uninterrupted cytoplasmic tyrosine kinase domain upstream of a carboxyterminal sterile alpha motif (SAM) domain. ephB family proteins bind ephrins of the B class, ligands that are also transmembrane spanning proteins capable of transmitting signals. ephB1 is expressed predominantly in developing neural structures in embryos, and in vascular epithelium of kidney, and other tissues. Upon binding to alternatively oligomerized ephrin B1, ephB1 signals regulation of cell attachment and cellcell assembly. Members of this protein family are implicated in neuronal and vascular cell targeting.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: EK6; ELK; EPH tyrosine kinase 2; EPH-like kinase 6; Ephrin type-B receptor 1; NET; Neuronally-expressed EPH-related tyrosine kinase; Tyrosine-protein kinase receptor EPH-2; unnamed protein product
Gene Aliases: 9330129L11; AW488255; C130099E04Rik; Cek6; ELK; Elkh; ENSMUSG00000074119; EPHB1; EPHT2; HEK6; NET
UniProt ID: (Human) P54762, (Mouse) Q8CBF3
Entrez Gene ID: (Human) 2047, (Mouse) 270190
nucleotide binding
protein kinase activity
protein tyrosine kinase activity
transmembrane receptor protein tyrosine kinase activity
ephrin receptor activity
transmembrane-ephrin receptor activity
protein binding
ATP binding
axon guidance receptor activity
kinase activity
transferase activity
macromolecular complex binding
transferase activity, transferring phosphorus-containing groups
protein complex binding
transmembrane signal receptor
angiogenesis
immunological synapse formation
cell adhesion
transmembrane receptor protein tyrosine kinase signaling pathway
nervous system development
axon guidance
skeletal muscle satellite cell activation
cell migration
cranial nerve development
optic nerve morphogenesis
hindbrain tangential cell migration
central nervous system projection neuron axonogenesis
neurogenesis
establishment of cell polarity
retinal ganglion cell axon guidance
cell-substrate adhesion
regulation of JNK cascade
protein autophosphorylation
ephrin receptor signaling pathway
camera-type eye morphogenesis
system development
modulation of synaptic transmission
detection of temperature stimulus involved in sensory perception of pain
positive regulation of synapse assembly
cell chemotaxis
dendritic spine development
dendritic spine morphogenesis
neural precursor cell proliferation
regulation of ERK1 and ERK2 cascade
negative regulation of skeletal muscle satellite cell proliferation
negative regulation of satellite cell differentiation
protein phosphorylation
phosphorylation
peptidyl-tyrosine phosphorylation
regulation of neuron death
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support