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Invitrogen
WASF3 Polyclonal Antibody
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FIGURE: 1 / 1
WASF3 Antibody (PA5-110576) in WB
Western blot analysis of WASF3 in extracts of various cell lines. Samples were incubated with WASF3 Polyclonal antibody (Product # PA5-110576) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-110576
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 200-300 of human WASF3 (NP_001278894.1).
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.71 mg/mL
Amount
71 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: RTRKEEWERR KMGIEFMSDA KKLEQAGSAK EDRVPSGSHA SDVTDYSYPA TPNHSLHPQP VTPSYAAGDV PPHGPASQAA EHEYRPPSAS ARHMALNRPQ Q
Target Information
WASP (for Wiskott-Aldrich syndrome protein) and N-WASP are downstream effectors of Cdc42 that are implicated in actin polymerization and cytoskeletal organization. The WASP family also includes VASP (vasodilator-stimulated phosphoprotein) and Mena (for mammalian enabled protein), which accumulate at focal adhesions and are also involved in the regulation of the actin cytoskeleton. The WAVE proteins are related to the WASP family proteins and are likewise involved in mediating actin reorganization downstream of the Rho family of small GTPases. The two protein homologs WAVE1 and WAVE2 specifically regulate membrane ruffling by inducing the formation of actin filament clusters in response to GTP binding and activating Rac. The WAVE proteins mediate this actin polymerization by cooperating with the Arp2/3 complex, a nucleation core, and thereby promoting the formation of actin filaments. WAVE1, which is also designated SCAR (for suppressor of cAR), is expressed primarily in the brain, while WAVE2 is widely expressed with the expression highest in peripheral blood leukocytes. WAVE3 forms a multiprotein complex that links receptor kinases and actin. Binding to actin occurs through a C-terminal verprolin homology domain in all family members. The multiprotein complex serves to tranduce signals that involve changes in cell shape, motility or function.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: actin polymerization protein; protein WAVE-3; unnamed protein product; verprolin homology domain-containing protein 3; WAS protein family member 3; WAS protein family, member 3; WASP family protein member 3; WASP family Verprolin-homologous protein 3; WASP-family protein; wiskott-Aldrich syndrome protein family member 3
Gene Aliases: Brush-1; SCAR3; WAVE3
UniProt ID: (Human) Q9UPY6, (Mouse) Q8VHI6
Entrez Gene ID: (Human) 10810, (Rat) 682937, (Mouse) 245880
oligodendrocyte development
lamellipodium assembly
actin cytoskeleton organization
positive regulation of myelination
modification of postsynaptic actin cytoskeleton
positive regulation of Arp2/3 complex-mediated actin nucleation
actin filament polymerization
protein-containing complex assembly
cytoskeleton organization
regulation of cell shape
lamellipodium organization
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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