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Invitrogen
beta-hydroxybutyryl-Histone H1.3 (Lys75) Polyclonal Antibody
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FIGURE: 1 / 2
beta-hydroxybutyryl-Histone H1.3 (Lys75) Antibody (PA5-112459) in ICC/IF
Immunocytochemichal analysis of beta-hydroxybutyryl-Histone H1.3 (Lys75) in Hela cells using a Polyclonal antibody (Product # PA5-112459) at a dilution of 1:10. Hela cells (treated with 50mM sodium 3-hydroxybutyrate for 4h) performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibo... View More
Product Details
PA5-112459
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Peptide sequence around site of beta-hydroxybutyryl-Lys (75) derived from Human Histone H1.3
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.13 mg/mL
Amount
13 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.03% ProClin 300
Storage conditions
-20°C or -80°C if preferred
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
Histones are basic nuclear proteins responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H1 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. [provided by RefSeq].
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: H1 histone family, member 3; histone 1, H1d; histone cluster 1 H1 family member d; histone cluster 1, H1d; Histone H1.3; Histone H1c; Histone H1s-2; unnamed protein product
Gene Aliases: H1-3; H1.3; H1D; H1F3; H1s-2; HIST1H1D
UniProt ID: (Human) P16402
Entrez Gene ID: (Human) 3007
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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