Search
Invitrogen
EHMT1 Polyclonal Antibody
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
Additional Information:
{{banner.description}}
FIGURE: 1 / 2
EHMT1 Antibody (PA5-114733) in ICC/IF
Immunocytochemistry analysis of EHMT1 in A549 cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-114733). Secondary staining was applied with mouse anti-beta tubulin (1 hr at 37°, AlexaFluor 594 conjugated goat anti-rabbit IgG (Red), AlexaFluor 488 conjugated goat anti-mouse IgG (Green) and DAPI.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-114733
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human EHMT1(Accession Q9H9B1), corresponding to amino acid residues C842-L892.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total EHMT1.
Target Information
EHMT1, also known as EUHMTASE1, is a histone methyltransferase. This protein methylates 'Lys-9' of histone H3 in vitro. H3 'Lys-9' methylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. EHMT1 is Probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. During G0 phase, it probably contributes to silencing of MYC- and E2F-responsive genes, suggesting a role in the G0/G1 transition of the cell cycle.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: EHMT1 intronic transcript 1; Eu-HMTase1; euchromatic histone-lysine N-methyltransferase 1; G9a-like protein 1; H3-K9-HMTase 5; histone H3-K9 methyltransferase 5; histone-lysine N-methyltransferase, H3 lysine-9 specific 5; lysine N-methyltransferase 1D; unnamed protein product
Gene Aliases: 9230102N17Rik; D330003E03; EHMT1-IT1; Eu-HMTase1; EUHMTASE1; FP13812; GLP; GLP1; KLEFS1; KMT1D; mKIAA1876
UniProt ID: (Human) Q9H9B1, (Mouse) Q5DW34
Entrez Gene ID: (Human) 79813, (Mouse) 77683
transcription corepressor binding
p53 binding
protein binding
methyltransferase activity
zinc ion binding
protein-lysine N-methyltransferase activity
histone methyltransferase activity
histone methyltransferase activity (H3-K9 specific)
histone methyltransferase activity (H3-K27 specific)
histone H3 methyltransferase activity
histone H3K9me2 methyltransferase activity
histone H3K9 monomethyltransferase activity
histone H3K9 trimethyltransferase activity
C2H2 zinc finger domain binding
histone H3K9 dimethyltransferase activity
negative regulation of transcription from RNA polymerase II promoter
chromatin organization
methylation-dependent chromatin silencing
peptidyl-lysine monomethylation
peptidyl-lysine dimethylation
regulation of gene expression, epigenetic
negative regulation of transcription, DNA-templated
regulation of embryonic development
brown fat cell differentiation
response to fungicide
positive regulation of cold-induced thermogenesis
beige fat cell differentiation
negative regulation of white fat cell differentiation
protein stabilization
negative regulation of muscle cell differentiation
facultative heterochromatin formation
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support