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Invitrogen
Phospho-EphA5 (Tyr650) Polyclonal Antibody
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FIGURE: 1 / 1
Phospho-EphA5 (Tyr650) Antibody (PA5-118755) in WB
Western blot analysis of Phospho-EphA5 (Tyr650) in UV treated MCF7 cells, LPS treated RAW264.7 cells, LPS treated C6 cells, LPS treated HepG2 cells using a Phospho-EphA5 (Tyr650) polyclonal antibody (Product # PA5-118755). Lane 1:UV treated MCF7 cells, blocked with antigen-specific peptides, Lane 2: UV treated MCF7 cells, Lane 3: RAW264.7 cells LPS, Lane 4: C6 cells LPS, Lane 5: HepG2 cells lps.
Product Details
PA5-118755
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human EPHA5(Accession P54756), corresponding to amino acid residues around phosphorylated Tyr650.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
sequential chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of EPHA5 only when phosphorylated at Tyr650.
Target Information
EPHA5 belongs to the ephrin receptor subfamily of the protein tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediated developmental events, particularly in the nervous system. Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. The tyrosine kinase (TK) group is mainly involved in the regulation of cell-cell interactions such as differentiation, adhesion, motility and death. There are currently about 90 TK genes sequenced, 58 are of receptor protein TK (e. g. EGFR, EPH, FGFR, PDGFR, TRK, and VEGFR families), and 32 of cytosolic TK (e. g. ABL, FAK, JAK, and SRC families).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ARCC2; Brain-specific kinase; CEK-7; CTPA; CTPP1; ECK; eck-like sequence 1; EHK-1; EK7; epa5; EPH homology kinase 1; EPH-like kinase 7; Ephrin A2; Ephrin A5; Ephrin type-A receptor 5; EphrinA2; EphrinA5; epididymis secretory sperm binding protein; receptor protein-tyrosine kinase HEK7; tyrosine-protein kinase receptor EHK-1
Gene Aliases: AI854630; AW125296; BSK; CEK7; EHK-1; EHK1; EK7; Ekh1; Els1; Els1.; EPHA5; HEK7; Rek7; TYRO4
UniProt ID: (Human) P54756, (Mouse) Q60629, (Rat) P54757
Entrez Gene ID: (Human) 2044, (Mouse) 13839, (Rat) 79208
nucleotide binding
protein kinase activity
protein tyrosine kinase activity
transmembrane receptor protein tyrosine kinase activity
ephrin receptor activity
GPI-linked ephrin receptor activity
transmembrane-ephrin receptor activity
protein binding
ATP binding
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
growth factor receptor binding
transmembrane signal receptor
transmembrane receptor protein tyrosine kinase signaling pathway
nervous system development
axon guidance
hippocampus development
positive regulation of CREB transcription factor activity
regulation of actin cytoskeleton organization
regulation of GTPase activity
ephrin receptor signaling pathway
neuron development
regulation of insulin secretion involved in cellular response to glucose stimulus
protein phosphorylation
positive regulation of phosphatidylinositol 3-kinase signaling
phosphorylation
peptidyl-tyrosine phosphorylation
cAMP-mediated signaling
negative regulation of synapse assembly
dendritic spine morphogenesis
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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