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          • Primary Antibodies ›
          • PKC Pan Antibodies

          Invitrogen

          PKC Pan Polyclonal Antibody

          View all (9) PKC Pan antibodies

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          Cite PKC Pan Polyclonal Antibody

          Additional Information:
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          • Antibody Testing Data (2)
          PKC Pan Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          PKC Pan Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 2

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          PKC Pan Antibody (PA5-120593) in ICC/IF

          Immunofluorescence analysis of PKC Pan in NIH-3T3 cells. Samples were incubated with PKC Pan Polyclonal antibody (Product # PA5-120593) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          View Product

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          PKC Pan Antibody in Immunocytochemistry (ICC/IF)
          PKC Pan Antibody in Western Blot (WB)

          Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

          Product Details

          PA5-120593

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:2,000
          -

          Immunocytochemistry (ICC/IF)

          1:50-1:200
          -

          ELISA (ELISA)

          1 µg/mL
          -
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          A synthetic peptide corresponding to a sequence within amino acids 572-672 of human PRKCA (NP_0027282)

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.27 mg/mL

          Amount

          27 µg

          Purification

          Affinity Chromatography

          Storage buffer

          PBS, pH 7.3, with 50% glycerol

          Contains

          0.02% sodium azide

          Storage conditions

          -20°C, Avoid Freeze/Thaw Cycles

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2914165

          Product Specific Information

          Positive test controls include: HeLa, Jurkat, 293T, Mouse brain, Rat brain.

          Immunogen sequence: MTKHPAKRLG CGPEGERDVR EHAFFRRIDW EKLENREIQP PFKPKVCGKG AENFDKFFTR GQPVLTPPDQ LVIANIDQSD FEGFSYVNPQ FVHPILQSAV

          Target Information

          Members of the protein kinase C (PKC) family play a key regulatory role in a variety of cellular functions including cell growth and differentiation, gene expression, hormone secretion and membrane function. PKCs were originally identified as serine/threonine protein kinases whose activity was dependent on calcium and phospholipids. Diacylglycerols (DAG) and tumor promoting phorbol esters bind to and activate PKC. PKCs can be subdivided into at least two major classes including conventional (c) PKC isoforms ( alpha, betaI, betaII and gamma) and novel (n) PKC isoforms ( delta, epsilon, zeta, eta and theta). Patterns of expression for each PKC isoform differ among tissues and PKC family members exhibit clear differences in their cofactor dependencies. For instance, the kinase activities of nPKC delta and epsilon are independent of Ca2+.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          References (0)

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          Cite this product

          Bioinformatics

          Protein Aliases: AAG6; aging-associated gene 6; alternatively spliced; aPKC-lambda/iota; atypical protein kinase C; atypical protein kinase C-lambda/iota; nPKC-delta; nPKC-epsilon; nPKC-eta; nPKC-iota; nPKC-theta; nPKC-zeta; PCK epsilon; PKC epsilon; PKC gamma; PKC lambda; PKC theta; PKC zeta; PKC-A; PKC-alpha; PKC-B; PKC-beta; PKC-gamma; PKC-L; PKCdelta; PKCdeltaVIII; PRKC-lambda/iota; Prkce; diacylglycerol sensitive, calcium insensitive ser/thr kinase; protein kinase; protein kinase C alpha-polypeptide; protein kinase C beta I; protein kinase C beta II; protein kinase C beta-1; protein kinase C beta-II; protein kinase C delta subspecies; protein kinase C delta VIII; protein kinase C epsilon subspecies; protein kinase C type I (gamma type); protein kinase C type II; protein kinase C type III; protein kinase C zeta subspecies; protein kinase C, beta 1; protein kinase C, beta 1 polypeptide; protein kinase C, delta IV; protein kinase C, delta V; protein kinase C, lambda; protein kinase C, zeta subspecies; protein kinase c-alpha; protein kinase C[d]; Protein uPEP2; tyrosine-protein kinase PRKCD; unnamed protein product

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          Gene Aliases: 14-3-3-zetaisoform; 2310021H13Rik; 5830406C15Rik; A130035A12Rik; AAG6; ALPS3; aPKClambda; aPKCzeta; CVID9; D14Ertd420e; DXS1179E; MAY1; mKIAA4165; nPKC-delta; nPKC-epsilon; nPKC-eta; nPKC-iota; nPKC-theta; nPKC-zeta; PKC; PKC-0; PKC-alpha; PKC-B; PKC-Beta; PKC-gamma; PKC-L; PKC-theta; PKC-ZETA; PKC2; PKC[d]; PKC[e]; PKCA; PKCalpha; PKCB; PKCbeta; PKCC; PKCD; PKCdelta; PKCE; PKCepsilon; PKCG; PKCgamma; Pkch; PKCI; PKCI(2); PKCI(3); PKCI+/-; PKCL; PKClambda; Pkcq; PKCtheta; Pkcz; Prkc; PRKCB1; PRKCB2; Prkcc; PRKCL; PRKCT; r14-3-3; RATPKCI; SCA14; uORF2; zetaPKC

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          UniProt ID: (Human) P17252, (Human) P05771, (Human) Q05655, (Human) Q02156, (Human) P05129, (Human) P24723, (Human) P41743, (Human) Q04759, (Human) Q05513, (Mouse) P20444, (Rat) P05696, (Rat) P68403, (Mouse) P68404, (Mouse) P28867, (Rat) P09215, (Rat) P09216, (Mouse) P16054, (Rat) P63319, (Mouse) P63318, (Rat) Q64617, (Mouse) P23298, (Mouse) Q62074, (Rat) F1M7Y5, (Mouse) Q02111, (Mouse) Q02956, (Rat) P09217

          View more View less

          Entrez Gene ID: (Human) 5578, (Human) 5579, (Human) 5580, (Human) 5581, (Human) 5582, (Human) 5583, (Human) 5584, (Human) 5588, (Human) 5590, (Mouse) 18750, (Rat) 24680, (Rat) 25023, (Mouse) 18751, (Mouse) 18753, (Rat) 170538, (Rat) 29340, (Mouse) 18754, (Rat) 24681, (Mouse) 18752, (Rat) 81749, (Mouse) 18755, (Mouse) 18759, (Rat) 84006, (Rat) 85420, (Mouse) 18761, (Mouse) 18762, (Rat) 25522

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          Function(s)
          protein kinase activity protein serine/threonine kinase activity protein kinase C activity calcium-dependent protein kinase C activity receptor binding integrin binding protein binding ATP binding zinc ion binding enzyme binding diacylglycerol binding histone kinase activity (H3-T6 specific) protein serine kinase activity chromatin binding transcription coactivator activity protein kinase C binding calcium channel regulator activity histone binding androgen receptor binding calcium-independent protein kinase C activity protein tyrosine kinase activity non-membrane spanning protein tyrosine kinase activity enzyme activator activity protein kinase binding protein tyrosine kinase activator activity insulin receptor substrate binding actin monomer binding receptor activator activity ethanol binding 14-3-3 protein binding protein serine/threonine/tyrosine kinase activity protein serine/threonine kinase inhibitor activity small GTPase binding translation regulator activity protein serine/threonine kinase binding phospholipid binding lipid binding TIR domain binding SH3 domain binding potassium channel regulator activity phospholipase binding macromolecular complex binding
          Process(es)
          phospholipase C-activating G-protein coupled receptor signaling pathway chemical synaptic transmission learning or memory response to toxic substance negative regulation of protein ubiquitination regulation of response to food positive regulation of mismatch repair intracellular signal transduction negative regulation of protein catabolic process regulation of circadian rhythm response to morphine negative regulation of neuron apoptotic process response to pain long-term synaptic potentiation innervation negative regulation of proteasomal protein catabolic process response to angiotensin response to psychosocial stress regulation of synaptic vesicle exocytosis response to hypoxia chromatin remodeling regulation of transcription from RNA polymerase II promoter protein phosphorylation calcium ion transport cellular calcium ion homeostasis phospholipase C-activating G-protein coupled acetylcholine receptor signaling pathway response to xenobiotic stimulus response to glucose regulation of signal transduction regulation of glucose transport negative regulation of glucose transport regulation of dopamine secretion dibenzo-p-dioxin metabolic process positive regulation of vascular endothelial growth factor receptor signaling pathway response to vitamin D regulation of growth B cell activation positive regulation of odontogenesis of dentin-containing tooth positive regulation of I-kappaB kinase/NF-kappaB signaling response to ethanol positive regulation of angiogenesis positive regulation of transcription, DNA-templated negative regulation of insulin receptor signaling pathway B cell receptor signaling pathway positive regulation of developmental process positive regulation of multicellular organismal process negative regulation of dopamine receptor signaling pathway protein kinase C signaling cellular response to carbohydrate stimulus positive regulation of ferroptosis regulation of secretion by cell protein targeting to membrane cytoskeleton organization positive regulation of neuron projection development vesicle-mediated transport cell migration establishment of cell polarity cellular response to insulin stimulus negative regulation of glial cell apoptotic process negative regulation of apoptotic process response to peptide hormone establishment or maintenance of epithelial cell apical/basal polarity cell-cell junction organization positive regulation of Notch signaling pathway positive regulation of glucose import secretion Golgi vesicle budding positive regulation of glial cell proliferation membrane organization cellular response to chemical stress response to interleukin-1 protein localization to plasma membrane regulation of postsynaptic membrane neurotransmitter receptor levels positive regulation of protein localization to plasma membrane positive regulation of endothelial cell apoptotic process apoptotic process signal transduction toxin catabolic process positive regulation of epithelial cell migration positive regulation of fibroblast migration regulation of metal ion transport positive regulation of actin filament polymerization lipopolysaccharide-mediated signaling pathway positive regulation of cytokinesis positive regulation of superoxide anion generation regulation of actin cytoskeleton organization TRAM-dependent toll-like receptor 4 signaling pathway Fc-gamma receptor signaling pathway involved in phagocytosis xenobiotic catabolic process positive regulation of wound healing negative regulation of sodium ion transmembrane transport regulation of cation transmembrane transport regulation of transcription, DNA-templated membrane protein ectodomain proteolysis regulation of G2/M transition of mitotic cell cycle regulation of vasoconstriction neuron differentiation positive regulation of telomere maintenance positive regulation of interleukin-17 production positive regulation of interleukin-2 production positive regulation of interleukin-4 production response to insulin positive regulation of T cell proliferation tissue regeneration positive regulation of protein import into nucleus respiratory burst positive regulation of protein secretion positive regulation of T cell activation positive regulation of filopodium assembly positive regulation of stress fiber assembly cell chemotaxis negative regulation of T cell apoptotic process regulation of platelet aggregation positive regulation of T-helper 17 type immune response positive regulation of T-helper 2 cell activation microtubule cytoskeleton organization positive regulation of cell-matrix adhesion cell surface receptor signaling pathway long-term memory positive regulation of cell proliferation peptidyl-serine phosphorylation negative regulation of protein complex assembly activation of protein kinase B activity positive regulation of interleukin-10 production positive regulation of interleukin-13 production positive regulation of interleukin-5 production positive regulation of phosphorylation positive regulation of T-helper 2 cell differentiation positive regulation of insulin receptor signaling pathway vesicle transport along microtubule positive regulation of synaptic transmission positive regulation of protein transport negative regulation of hydrolase activity membrane depolarization membrane hyperpolarization positive regulation of ERK1 and ERK2 cascade regulation of neurotransmitter receptor localization to postsynaptic specialization membrane positive regulation of smooth muscle cell-matrix adhesion neuron projection extension positive regulation of excitatory postsynaptic potential positive regulation of T-helper 2 cell cytokine production cellular response to DNA damage stimulus intrinsic apoptotic signaling pathway in response to oxidative stress peptidyl-threonine phosphorylation termination of signal transduction negative regulation of actin filament polymerization cellular response to UV neutrophil activation defense response to bacterium negative regulation of MAPK cascade regulation of mRNA stability positive regulation of DNA repair negative regulation of smoothened signaling pathway negative regulation of inflammatory response protein stabilization positive regulation of DNA metabolic process negative regulation of filopodium assembly cellular response to hydrogen peroxide cellular response to fatty acid cellular response to hydroperoxide negative regulation of platelet aggregation cellular senescence phospholipase C/protein kinase C signal transduction positive regulation of phospholipid scramblase activity cellular response to angiotensin regulation of ceramide biosynthetic process positive regulation of ceramide biosynthetic process positive regulation of glucosylceramide catabolic process positive regulation of sphingomyelin catabolic process response to oxidative stress immunoglobulin mediated immune response collagen metabolic process cellular response to oxidative stress B cell proliferation regulation of phosphorylation positive regulation of apoptotic process positive regulation of MAP kinase activity positive regulation of MAPK cascade post-translational protein modification protein autophosphorylation D-aspartate import positive regulation of apoptotic signaling pathway macrophage activation involved in immune response positive regulation of cell-substrate adhesion negative regulation of mitochondrial membrane potential regulation of lipid metabolic process positive regulation of insulin secretion positive regulation of synaptic transmission, GABAergic locomotory exploration behavior cellular response to platelet-derived growth factor stimulus regulation of peptidyl-tyrosine phosphorylation positive regulation of lipid catabolic process regulation of release of sequestered calcium ion into cytosol negative regulation of release of sequestered calcium ion into cytosol negative regulation of mitochondrial calcium ion concentration regulation of insulin secretion involved in cellular response to glucose stimulus positive regulation of mucus secretion cellular response to ethanol cellular response to prostaglandin E stimulus cellular response to hypoxia presynaptic modulation of chemical synaptic transmission response to mechanical stimulus cellular response to glucose starvation response to hydrogen peroxide response to amino acid response to phorbol 13-acetate 12-myristate regulation of cell growth axon guidance Fc-epsilon receptor signaling pathway response to reactive oxygen species negative regulation of protein phosphorylation positive regulation of protein phosphorylation positive regulation of endothelial cell proliferation regulation of the force of heart contraction chondrocyte differentiation desmosome assembly negative regulation of protein kinase activity regulation of muscle contraction negative regulation of cell proliferation positive regulation of signal transduction positive regulation of endothelial cell migration positive regulation of cardiac muscle hypertrophy negative regulation of translation central nervous system neuron axonogenesis signal transduction involved in regulation of gene expression positive regulation of cell migration neutrophil chemotaxis positive regulation of lipopolysaccharide-mediated signaling pathway response to estradiol peptidyl-serine autophosphorylation proteasome-mediated ubiquitin-dependent protein catabolic process protein kinase B signaling positive regulation of blood vessel endothelial cell migration positive regulation of cellular component biogenesis positive regulation of macrophage differentiation positive regulation of bone resorption positive regulation of cell adhesion positive regulation of exocytosis positive regulation of mitotic cell cycle negative regulation of glucose import positive regulation of smooth muscle cell proliferation positive regulation of inflammatory response induction of positive chemotaxis positive regulation of protein kinase B signaling positive regulation of synapse assembly renal sodium ion absorption intrinsic apoptotic signaling pathway positive regulation of angiotensin-activated signaling pathway negative regulation of cytokine production involved in inflammatory response positive regulation of dense core granule biogenesis chemosensory behavior regulation of cell communication regulation of signaling regulation of phagocytosis positive regulation of macrophage derived foam cell differentiation positive regulation of translation in response to stress cellular response to amino acid starvation positive regulation of keratinocyte differentiation positive regulation of B cell receptor signaling pathway regulation of bicellular tight junction assembly actin filament organization establishment of apical/basal cell polarity eye photoreceptor cell development regulation of exocytosis establishment of protein localization response to corticosterone mitotic nuclear envelope disassembly lipoprotein transport regulation of transport apoptotic signaling pathway positive regulation of adenylate cyclase-activating G protein-coupled receptor signaling pathway synaptic signaling via neuropeptide
          It has to be done as per old AB suggested Products section.

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