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Invitrogen
AES Polyclonal Antibody
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FIGURE: 1 / 1
AES Antibody (PA5-121149) in WB
Western blot analysis of AES in extracts of various cell lines. Samples were incubated with AES Polyclonal antibody (Product # PA5-121149) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-121149
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-264 of human AES (NP_945320.1).
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
8.47 mg/mL
Amount
847 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive test controls include: A-549, HeLa, U-251MG, Mouse liver. The target is usually found in the following locations: Nucleus.
Immunogen sequence: MCHKNGFPQE GGITAAFLQK RKLRLSKNHR PARAKVTEHV RGTRPGRATA GPAASTRAAG SLFFDRWGNR GPAGCRGSSH LPQQLKFTTS DSCDRIKDEF QLLQAQYHSL KLECDKLASE KSEMQRHYVM YYEMSYGLNI EMHKQAEIVK RLNGICAQVL PYLSQEHQQQ VLGAIERAKQ VTAPELNSII RQQLQAHQLS QLQALALPLT PLPVGLQPPS LPAVSAGTGL LSLSALGSQA HLSKEDKNGH DGDTHQEDDG EKSD
Target Information
Adhesion to extracellular matrix regulates cell survival through both integrin engagement and appropriate cell spreading. Anoikis is the molecular mechanism of apop-tosis induced by integrin detachment. Amino-terminal enhancer of split (AES) is a member of the Groucho/ transducin-like enhancer of split (TLE) family of transcriptional regulators, a group of transcriptional co-repressors that play important roles in neurogenesis, segmentation, and sex determination. AES forms a complex with Bit1 (Bcl-2 inhibitor of transcription 1), a mitochondrial protein that is released into the cytoplasm upon onset of apoptosis. It has been suggested that this complex turns off a survival-promoting gene transcription program controlled by the TLE protein family. Interestingly, apoptosis of cells transfected with AES and Bit1 could be inhibited if the cells were allowed to attach to fibronectin through the alpha5beta1 integrin suggesting that the Bit1-AES pathway contributing to anoikis is regulated by integrins, and in particular, the alpha5beta1 integrin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: amino-terminal enhancer of split; gp130-associated protein GAM; groucho-related protein 5; hGAM protein; homologous to Drosophila groucho gene product and human TLE gene products; homologous to Swiss-Prot accession number P16371; related to Drosophila groucho; unnamed protein product
Gene Aliases: AES; AES-1; AES-2; ESP1; GRG; Grg-5; GRG5
UniProt ID: (Human) Q08117, (Mouse) P63002
Entrez Gene ID: (Human) 166, (Mouse) 14797
negative regulation of transcription from RNA polymerase II promoter
regulation of transcription, DNA-templated
negative regulation of gene expression
negative regulation of transcription, DNA-templated
negative regulation of response to cytokine stimulus
response to interleukin-1
negative regulation of canonical Wnt signaling pathway
positive regulation of anoikis
skeletal system development
regulation of transcription from RNA polymerase II promoter
regulation of growth
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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