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          • Primary Antibodies ›
          • PLOD1 Antibodies

          Invitrogen

          PLOD1 Polyclonal Antibody

          View all (7) PLOD1 antibodies

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          Cite PLOD1 Polyclonal Antibody

          Additional Information:
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          • Antibody Testing Data (1)
          PLOD1 Antibody in Western Blot (WB)
          Group 53 Created with Sketch.
          PLOD1 Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          FIGURE: 1 / 1

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          PLOD1 Antibody (PA5-121155) in WB

          Western blot analysis of PLOD1 in extracts of various cell lines. Samples were incubated with PLOD1 Polyclonal antibody (Product # PA5-121155) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          PLOD1 Antibody in Western Blot (WB)

          Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

          PLOD1 Polyclonal Antibody

          Product Details

          PA5-121155

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:1,000
          -

          ELISA (ELISA)

          1 µg/mL
          -
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Recombinant protein (or fragment).
          3D Epitope / Immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          2.6 mg/mL

          Amount

          260 µg

          Purification

          Affinity Chromatography

          Storage buffer

          PBS, pH 7.3, with 50% glycerol

          Contains

          0.02% sodium azide

          Storage conditions

          -20°C, Avoid Freeze/Thaw Cycles

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2914727

          Product Specific Information

          Positive test controls include: HepG2, A-431, U-251MG, Mouse kidney, Mouse heart. The target is usually found in the following locations: Lumenal side, Peripheral membrane protein, Rough endoplasmic reticulum membrane.

          Immunogen sequence: KGDAKPEDNL LVLTVATKET EGFRRFKRSA QFFNYKIQAL GLGEDWNVEK GTSAGGGQKV RLLKKALEKH ADKEDLVILF ADSYDVLFAS GPRELLKKFR QARSQVVFSA EELIYPDRRL ETKYPVVSDG KRFLGSGGFI GYAPNLSKLV AEWEGQDSDS DQLFYTKIFL DPEKREQINI TLDHRCRIFQ NLDGALDEVV LKFEMGHVRA RNLAYDTLPV LIHGNGPTKL QLNYLGNYIP RFWTFETGCT VCDEGLRSLK GIGDEALPTV LVGVFIE

          Target Information

          Forms hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens. These hydroxylysines serve as sites of attachment for carbohydrate units and are essential for the stability of the intermolecular collagen cross-links.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          References (0)

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          Cite this product

          Bioinformatics

          Protein Aliases: LH1; lysine hydroxylase; lysyl hydroxlase 1; lysyl hydroxylase 1; procollagen lysyl hydroxylase 1; procollagen-lysine 1, 2-oxoglutarate 5-dioxygenase 1; procollagen-lysine, 2-oxoglutarate 5-dioxygenase (lysine hydroxylase, Ehlers-Danlos syndrome type VI); unnamed protein product

          View more View less

          Gene Aliases: 2410042F05Rik; EDS6; EDSKCL1; LH; LH1; LLH; PLOD

          View more View less

          UniProt ID: (Human) Q02809, (Mouse) Q9R0E2

          View more View less

          Entrez Gene ID: (Human) 5351, (Mouse) 18822, (Rat) 116552

          View more View less

          Function(s)
          iron ion binding protein binding procollagen-lysine 5-dioxygenase activity oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen L-ascorbic acid binding procollagen glucosyltransferase activity ferrous iron binding peptide binding
          Process(es)
          response to hypoxia protein O-linked glycosylation epidermis development collagen fibril organization collagen biosynthetic process peptidyl-lysine hydroxylation cellular response to hormone stimulus
          It has to be done as per old AB suggested Products section.

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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

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          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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