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Invitrogen
ATG9A Polyclonal Antibody
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FIGURE: 1 / 1
ATG9A Antibody (PA5-121164) in WB
Western blot analysis of ATG9A in extracts of Mouse liver. Samples were incubated with ATG9A Polyclonal antibody (Product # PA5-121164) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 5s.
Product Details
PA5-121164
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 180-290 of human ATG9A (NP_076990.4).
Conjugate
Form
Liquid
Concentration
1.05 mg/mL
Amount
105 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive test controls include: Mouse liver. The target is usually found in the following locations: Cytoplasmic vesicle, Endoplasmic reticulum membrane, Golgi apparatus, Late endosome membrane, Multi-pass membrane protein, autophagosome membrane, trans-Golgi network membrane.
Immunogen sequence: WQEVQARIVQ TQKEHQICIH KRELTELDIY HRILRFQNYM VALVNKSLLP LRFRLPGLGE AVFFTRGLKY NFELILFWGP GSLFLNEWSL KAEYKRGGQR LELAQRLSNR I
Target Information
Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). Apg9 plays a direct role in the formation of the cytoplasm to vacuole targeting and autophagic vesicles, possibly serving as a marker for a specialized compartment essential for these vesicle-mediated alternative targeting pathways.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Apg9; APG9 autophagy 9-like 1; APG9-like 1; Apg911; APG9A; ATG 9A; ATG9 autophagy related 9 homolog A; autophagy 9-like 1 protein; autophagy protein 9; autophagy-related 9-like 1; autophagy-related 9A; Autophagy-related protein 9A; FLJ22169; mATG9; unnamed protein product
Gene Aliases: APG9L1; Atg9; ATG9A; Atg9l1; AU019532; mATG9; MGD3208; RGD1310450
UniProt ID: (Human) Q7Z3C6, (Mouse) Q68FE2, (Rat) Q5FWU3
Entrez Gene ID: (Human) 79065, (Mouse) 245860, (Rat) 363254
autophagosome assembly
mitophagy
transport
autophagy
protein transport
negative regulation of interferon-beta production
protein localization to Golgi apparatus
protein localization to pre-autophagosomal structure
piecemeal microautophagy of nucleus
late nucleophagy
innate immune response
mitophagy
lipid transport
negative regulation of macrophage cytokine production
phospholipid scrambling
bone morphogenesis
reticulophagy
programmed necrotic cell death
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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