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Invitrogen
Phospho-Coronin 1B (Ser2) Polyclonal Antibody
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FIGURE: 1 / 2
Phospho-Coronin 1B (Ser2) Antibody (PA5-143670) in ICC/IF
Immunocytochemistry analysis of Phospho-Coronin 1B (Ser2) in rabbit spleen fibroblasts treated with Calyculin A. The cells were labeled with rabbit polyclonal Coronin-1B (C-terminus) and Phospho-Coronin 1B (Ser2) polyclonal antibody (Product # PA5-143670), then detected using appropriate secondary antibodies conjugated to Cy3. The antibodies were used in the absence (left) or presence (right) of their respe... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-143670
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Coronin-1B (Ser-2) synthetic peptide (coupled to carrier protein) corresponds to amino acids surrounding serine 2 in human coronin-1B. This sequence has high homology to the conserved site in rat and mouse coronin-1B and in coronin-1A. However, this sequence has low homology to the conserved region in other coronins.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.25 mg/mL
Amount
25 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 1mg/mL BSA, 50% glycerol
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
The antibody detects a 60 kDa* band corresponding to Coronin-1B on SDS-PAGE immunoblots of human A431 or HeLa cells treated with Calyculin A. This band is not observed in control cells and the band observed in Calyculin A-treated cells can be removed with lambda phosphatase treatment.
Target Information
Members of the coronin family, such as CORO1B, are WD repeat-containing actin-binding proteins that regulate cell motility.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: coronin 1b; coronin, actin binding protein, 1B; coronin, actin-binding protein, 1B; coronin-2; unnamed protein product
Gene Aliases: CORONIN-2
UniProt ID: (Human) Q9BR76, (Rat) O89046, (Mouse) Q9WUM3
Entrez Gene ID: (Human) 57175, (Rat) 29474, (Mouse) 23789
actin filament organization
cell migration
actin cytoskeleton organization
ruffle organization
regulation of Arp2/3 complex-mediated actin nucleation
negative regulation of Arp2/3 complex-mediated actin nucleation
endothelial cell chemotaxis
cellular response to platelet-derived growth factor stimulus
wound healing
actin filament bundle assembly
negative regulation of cellular component organization
negative regulation of smooth muscle cell chemotaxis
actin filament branching
protein localization to cell leading edge
positive regulation of lamellipodium morphogenesis
intracellular signal transduction
negative regulation of lamellipodium morphogenesis
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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