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Invitrogen

Phospho-PDPK1 (Tyr9) Polyclonal Antibody

View all (45) PDPK1 antibodies

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Product Details

PA5-143740

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500
-

Immunocytochemistry (ICC/IF)

1:50
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Phospho-PDK1 (Tyr-9) synthetic peptide (coupled to carrier protein) corresponds to amino acids surrounding tyrosine 9 in human PDK1. This sequence is highly conserved in rat and mouse PDK1.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA, 50% glycerol

Contains

0.05% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2942968

Product Specific Information

This antibody was cross-adsorbed to phospho-tyrosine coupled to agarose, then affinity purified using phospho-PDK1 (Tyr-9) peptide. The purified antibody detects a 65 kDa* protein corresponding to the apparent molecular mass of PDK1 on SDS-PAGE immunoblots of human A431 cells treated with EGF, IGF-1, or pervanadate, but not in untreated cells. PDK1 (Tyr-9) reactivity was also observed in adult mouse brain.

Target Information

PDPK1 (3 Phosphoinositide Dependent Protein Kinase 1) phosphorylates AGC kinases. PDPK1 activates conventional PKC and PKC zeta through phosphorylation of critical threonine residues in the activation loop. PDPK1 also phosphorylates Protein Kinase B (PKB) at threonine 308 in the presence of phosphatidylinositol-3,4,5-trisphosphate. Active Akt inactivates Glycogen Synthase Kinase 3 (GSK3), eventually leading to the dephosphorylation and activation of glycogen synthase, and the stimulation of glycogen synthesis. Because of the role that PDPK1 plays in insulin-induced glycogen synthesis and PKC activation, it is a potentially important target for metabolic drug research.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 3-phosphoinositide dependent protein kinase-1; 3-phosphoinositide-dependent protein kinase 1; 3-phosphoinositide-dependent protein kinase 2 pseudogene; HGNC:8809; hPDK1; isoenzyme 1; kinase isoenzyme 1; lipoamide; mPDK1; OTTHUMP00000174525; OTTHUMP00000205076; PDPK1; PkB kinase; PkB kinase like gene 1; PkB-like 1; predicted protein of HQ0461; Protein kinase B kinase; Putative 3-phosphoinositide-dependent protein kinase 2; unnamed protein product

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Gene Aliases: PDK1; PDPK1; PDPK2; PDPK2P; PRO0461

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UniProt ID: (Human) O15530, (Mouse) Q9Z2A0, (Rat) O55173

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Entrez Gene ID: (Human) 5170, (Mouse) 18607, (Rat) 81745

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Function(s)
nucleotide binding protein kinase activity protein serine/threonine kinase activity 3-phosphoinositide-dependent protein kinase activity protein binding ATP binding phospholipase activator activity kinase activity transferase activity phospholipase binding protein serine kinase activity insulin receptor binding transferase activity, transferring phosphorus-containing groups protein kinase binding non-receptor serine/threonine protein kinase
Process(es)
type B pancreatic cell development protein phosphorylation hyperosmotic response epidermal growth factor receptor signaling pathway insulin receptor signaling pathway regulation of endothelial cell migration negative regulation of cardiac muscle cell apoptotic process cell migration calcium-mediated signaling actin cytoskeleton organization negative regulation of transforming growth factor beta receptor signaling pathway T cell costimulation cellular response to insulin stimulus negative regulation of toll-like receptor signaling pathway intracellular signal transduction negative regulation of apoptotic process regulation of I-kappaB kinase/NF-kappaB signaling regulation of mast cell degranulation protein kinase B signaling positive regulation of blood vessel endothelial cell migration positive regulation of angiogenesis protein autophosphorylation insulin-like growth factor receptor signaling pathway positive regulation of release of sequestered calcium ion into cytosol positive regulation of protein kinase B signaling cellular response to epidermal growth factor stimulus extrinsic apoptotic signaling pathway vascular endothelial cell response to laminar fluid shear stress intracellular signaling cassette positive regulation of protein localization to plasma membrane positive regulation of sprouting angiogenesis positive regulation of vascular endothelial cell proliferation negative regulation of endothelial cell apoptotic process transcription, DNA-templated regulation of transcription, DNA-templated negative regulation of protein kinase activity signal transduction positive regulation of phospholipase activity phosphorylation peptidyl-threonine phosphorylation activation of protein kinase B activity focal adhesion assembly positive regulation of establishment of protein localization to plasma membrane
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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