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          • Primary Antibodies ›
          • C4orf23 Antibodies

          Invitrogen

          C4orf23 Polyclonal Antibody

          View all (4) C4orf23 antibodies

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          Cite C4orf23 Polyclonal Antibody

          Additional Information:
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          • Antibody Testing Data (2)
          C4orf23 Antibody in Western Blot (WB)
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          C4orf23 Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          FIGURE: 1 / 2

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          C4orf23 Antibody (PA5-144043) in WB

          Western blot analysis of TRMT44 using TRMT44 antibody (Product # PA5-144043). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 µg of sample under reducing conditions. Lane 1: human K562 whole cell lysates; Lane 2: human MOLT-4 whole cell lysates; Lane 3: human U251 whole cell lysates; Lane 4: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. B... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          C4orf23 Antibody in Western Blot (WB)
          C4orf23 Antibody in Flow Cytometry (Flow)

          Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

          C4orf23 Polyclonal Antibody

          Product Details

          PA5-144043

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          0.25-0.5 µg/mL
          -

          Flow Cytometry (Flow)

          1-3 µg/1x10^6 cells
          -

          ELISA (ELISA)

          0.1-0.5 µg/mL
          -
          Product Specifications

          Species Reactivity

          Human

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          E.coli-derived human TRMT44 recombinant protein (Position: M1-S757).
          3D Epitope / Immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Lyophilized

          Concentration

          500 µg/mL

          Amount

          100 µg

          Purification

          Antigen affinity chromatography

          Storage buffer

          PBS with 4mg trehalose

          Contains

          no preservative

          Storage conditions

          -20°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_3075257

          Product Specific Information

          Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.

          Positive Control - WB: human K562 whole cell, human MOLT-4 whole cell, human U251 whole cell, human Hela whole cell. Flow: 293T cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

          Target Information

          Probable adenosyl-L-methionine (AdoMet)-dependent tRNA (uracil-O(2)-)-methyltransferase.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          References (0)

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          Cite this product

          Bioinformatics

          Protein Aliases: methyltransferase like 19; methyltransferase-like protein 19; putative tRNA (uracil-O(2)-)-methyltransferase; unnamed protein product

          View more View less

          Gene Aliases: C4orf23; METTL19; TRM44

          View more View less

          UniProt ID: (Human) Q8IYL2

          View more View less

          Entrez Gene ID: (Human) 152992

          View more View less

          Function(s)
          methyltransferase activity tRNA (uracil) methyltransferase activity metal ion binding tRNA(Ser) (uridine(44)-2'-O-)-methyltransferase activity
          Process(es)
          tRNA methylation
          It has to be done as per old AB suggested Products section.

          Disclaimer

          Close

          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

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          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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