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Invitrogen
alpha Actinin 1 Polyclonal Antibody
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FIGURE: 1 / 12
alpha Actinin 1 Antibody (PA5-17308) in ICC/IF
Immunofluorescent analysis of Alpha-Actinin in NIH/3T3 cells using an Alpha-Actinin polyclonal antibody (Product # PA5-17308) (green). Actin filaments are labeled with a fluorescent red phalloidin. DNA is labeled using a fluorescent blue dye.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-17308
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Hamster,
Human,
Mouse,
Non-human primate,
Rat
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to carboxy-terminal residues of human a-actinin-1
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
5 µg/mL
Amount
0.5 µg
Purification
Affinity chromatography
Storage buffer
0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
It is not recommended to aliquot this antibody.
Target Information
Alpha Actinin 1 belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, cytoskeletal, alpha actinin isoform and maps to the same site as the structurally similar erythroid beta spectrin gene. Three transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: actinin 1 smooth muscle; actinin, alpha 1; alpha actinin 1a; Alpha-actinin-1; bs-alpha actinin 1; epididymis secretory sperm binding protein; F-actin cross-linking protein; FLJ40884; FLJ54432; non-muscle alpha-actinin-1; unnamed protein product
Gene Aliases: 3110023F10Rik; Actn1a; BDPLT15; I79_004057
UniProt ID: (Human) P12814, (Mouse) Q7TPR4, (Rat) Q9Z1P2
Entrez Gene ID: (Human) 87, (Mouse) 109711, (Chinese hamster) 100755831, (Rat) 81634
transcription coactivator activity
double-stranded RNA binding
integrin binding
calcium ion binding
protein binding
cytoskeletal protein binding
vinculin binding
identical protein binding
protein homodimerization activity
ion channel binding
actin filament binding
structural constituent of postsynapse
cytoskeletal regulatory protein binding
protein domain specific binding
actin filament organization
actin cytoskeleton organization
platelet formation
cortical cytoskeleton organization
platelet morphogenesis
positive regulation of transcription, DNA-templated
focal adhesion assembly
actin filament bundle assembly
actin filament network formation
muscle cell development
postsynapse organization
platelet activation
regulation of apoptotic process
anatomical structure formation involved in morphogenesis
platelet aggregation
fibril organization
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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