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Invitrogen
TLR3 Polyclonal Antibody
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FIGURE: 1 / 1
TLR3 Antibody (PA5-23349) in WB
Western blot analysis of TLR3 in various lysates. Samples were incubated with TLR3 polyclonal antibody (Product # PA5-23349) followed by Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution. Cell lysate in the (1) absence and (2) presence of immunizing peptide and (3) 3T3 cell lysate.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-23349
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A portion of amino acids 750-800 of human TLR3.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 0.05% BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
TLR3 (Toll-like receptor 3, CD283) is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from drosophila to humans and share structural and functional similarities. TLRs recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. TLR3 is most abundantly expressed in placenta and pancreas, and is restricted to the dendritic subpopulation of the leukocytes. TLR3 recognizes dsRNA associated with viral infection, and induces the activation of NF-kappaB and the production of type I interferons. Further, TLR3 may play a role in host defense against viruses, and the use of alternative polyadenylation sites to generate different length transcripts has been noted for the TLR3 gene. Like its counterparts in Drosophila, TLRs signal through adaptor molecules and could constitute an important and unrecognized component of innate immunity in humans. The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition. TLRs characterized so far activate the MyD88/interleukin-1 receptor-associated kinase (IRAK) signaling pathway. TLR3 is the only human TLR which does not utilize MyD88 as an adaptor molecule instead utilizing TRIF in a signaling path which results in downstream activation of IRF-3 and NF-kB. TLR3 activation leads not only to Type I interferon induction but also to other inflammatory cytokines which result in DC maturation. TLR3 is known to recognize viral double-stranded (ds) RNA, a molecular pattern associated with viral infection. Recently it has been shown to recognize viruses such as Influenza A and West Nile Virus and can mediate entry of at least West Nile Virus.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: TLR 3; TLR3; unnamed protein product
Gene Aliases: CD283; IIAE2; IMD83
UniProt ID: (Human) O15455, (Mouse) Q99MB1
Entrez Gene ID: (Human) 7098, (Mouse) 142980
MAPK cascade
toll-like receptor signaling pathway
regulation of immune system process
hyperosmotic response
signal transduction
activation of NF-kappaB-inducing kinase activity
JNK cascade
detection of virus
positive regulation of gene expression
positive regulation of chemokine production
positive regulation of interferon-alpha production
positive regulation of interferon-beta production
positive regulation of interferon-gamma production
positive regulation of interleukin-12 production
positive regulation of interleukin-6 production
positive regulation of interleukin-8 production
positive regulation of tumor necrosis factor production
toll-like receptor 3 signaling pathway
intracellular signal transduction
defense response to bacterium
positive regulation of I-kappaB kinase/NF-kappaB signaling
response to exogenous dsRNA
response to dsRNA
innate immune response
negative regulation of osteoclast differentiation
positive regulation of angiogenesis
positive regulation of transcription from RNA polymerase II promoter
positive regulation of JNK cascade
positive regulation of inflammatory response
defense response to virus
cellular response to mechanical stimulus
inflammatory response to wounding
extrinsic apoptotic signaling pathway
necroptotic signaling pathway
cellular response to virus
positive regulation of cytokine production involved in inflammatory response
microglial cell activation
regulation of dendritic cell cytokine production
defense response
inflammatory response
response to virus
positive regulation of type I interferon production
regulation of toll-like receptor 3 signaling pathway
positive regulation of type III interferon production
cellular response to exogenous dsRNA
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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