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Invitrogen
GLD2 Polyclonal Antibody
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FIGURE: 1 / 5
GLD2 Antibody (PA5-25015) in ICC/IF
Immunocytochemistry analysis of GLD2 in MCF-7 cells. Samples were incubated with GLD2 polyclonal antibody (Product # PA5-25015) using a dilution of 1:25 followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG at a dilution of 1:400 (green). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-25015
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
KLH conjugated synthetic peptide between 58-87 amino acids from the N-terminal region of human GLD2
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.45 mg/mL
Amount
180 µg
Purification
Protein A, Antigen affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody is predicted to react with bovine based on sequence homology.
Target Information
GLD2 is cytoplasmic poly(A) RNA polymerase that adds successive AMP monomers to the 3'-end of specific RNAs, forming a poly(A) tail. In contrast to the canonical nuclear poly(A) RNA polymerase, it only adds poly(A) to selected cytoplasmic mRNAs. GLD2 does not play a role in replication-dependent histone mRNA degradation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: cytoplasmic poly(A) polymerase; PAP associated domain containing 4; PAP-associated domain-containing protein 4; poly(A) RNA polymerase D4, non-canonical; terminal uridylyltransferase 2; TUTase 2; unnamed protein product
Gene Aliases: 8030446C20Rik; APD4; GLD-2; GLD2; PAPD4; TUT2
UniProt ID: (Human) Q6PIY7, (Mouse) Q91YI6
Entrez Gene ID: (Human) 167153, (Mouse) 100715
hematopoietic progenitor cell differentiation
posttranscriptional regulation of gene expression
RNA metabolic process
mRNA 3'-end processing
histone mRNA catabolic process
positive regulation of protein targeting to membrane
postsynaptic modulation of chemical synaptic transmission
RNA polyadenylation at postsynapse
regulation of translation at postsynapse
target-directed miRNA degradation
negative regulation of miRNA catabolic process
regulation of gene expression
RNA 3'-end processing
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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