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Invitrogen
AANAT Polyclonal Antibody
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FIGURE: 1 / 2
AANAT Antibody (PA5-26631) in IHC
Immunofluorescence was performed on sections of adult rat pineal. Tissue sections on slides were probed for 24 h at 4°C in a humidified chamber with rabbit anti-AANAT (Product # PA5-26631) at an antibody concentration of 1-2 µg/mL diluted in 50 mM Tris-HCl, pH 7.4, containing 1.5% NaCl, 0.3% Triton X-100 (TBST) and 4% normal goat serum. After overnight incubation, sections were washed extensively for 1 h in TBST, and detection of primary antibody was performed for 1.5 h at room temperature with AlexaFluor-488-conjugated donkey anti-rabbit ... View More
Product Details
PA5-26631
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
KLH conjugated synthetic peptide between 27-54 amino acids from the N-terminal region of human AANAT
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.5 mg/mL
Amount
200 µg
Purification
Protein A, Antigen affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
PA5-26631 has successfully been used to detect serotonin N-AT in both WB and IF applications.
Target Information
Arylalkylamine N-Acetyltransferase (AANAT) is the penultimate enzyme is melatonin synthesis. Hormonal melatonin has been implicated in sleep and circadian clock function. Large changes in the abundance of AANAT are believed to be responsible for the large day/night rhythms in melatonin synthesis in the pineal gland and retina. Most research shows that AANAT activity is regulated by controlling the steady-state levels of the protein. Recent research, however, suggests that another mechanism may also play a role in controlling melatonin production without altering AANAT protein and activity. This mechanism may explain the sudden physiological increase in circulating melatonin observed at the onset of the dark period in humans.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: AA-NAT; Aralkylamine N-acetyltransferase; arylalkylamine N-acetyltransferase; Serotonin acetylase; Serotonin N-acetyltransferase
Gene Aliases: AANAT; DSPS; SNAT
UniProt ID: (Human) Q16613
Entrez Gene ID: (Human) 15
N-terminal protein amino acid acetylation
circadian rhythm
response to light stimulus
photoperiodism
response to zinc ion
melatonin biosynthetic process
response to insulin
response to cytokine
response to prostaglandin E
indolalkylamine biosynthetic process
response to copper ion
rhythmic process
response to corticosterone
response to cAMP
response to calcium ion
cellular response to cAMP
response to peptide
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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