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Invitrogen
TOP2A Polyclonal Antibody
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Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-27248
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Topoisomerase II alpha. The exact sequence is proprietary.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
0.1M tris glycine, pH 7, with 10% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Recommended positive controls: 293T, A431, H1299.
Predicted reactivity: Pig (100%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Topo II alpha plays important roles in synthesis and transcription of DNA as well as chromosomal segregation during mitosis. It is reported to be a sensitive and specific marker of late S-, G2 and M-phases in transformed and developmentally regulated normal cellsTopo II alpha is also implicated in drug resistance of tumor cells.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DNA gyrase; DNA topoisomerase (ATP-hydrolyzing); DNA topoisomerase II (EC 5.99.1.3); DNA topoisomerase II, 170 kD; DNA topoisomerase II, alpha isozyme; EC 5.99.1.3; OTTHUMP00000207108; P11388-1; TOP2a/90; topoisomerase (DNA) II alpha 170kDa; topoisomerase ii alpha
Gene Aliases: TOP2; TOP2alpha; TOPIIA; TP2A
UniProt ID: (Human) P11388
Entrez Gene ID: (Human) 7153
magnesium ion binding
DNA binding
chromatin binding
RNA binding
DNA topoisomerase type II (ATP-hydrolyzing) activity
protein kinase C binding
protein binding
ATP binding
DNA-dependent ATPase activity
DNA binding, bending
protein homodimerization activity
ubiquitin binding
protein heterodimerization activity
resolution of meiotic recombination intermediates
sister chromatid segregation
DNA metabolic process
DNA topological change
chromatin organization
cellular response to DNA damage stimulus
chromosome segregation
female meiotic division
apoptotic chromosome condensation
regulation of circadian rhythm
positive regulation of apoptotic process
positive regulation of single stranded viral RNA replication via double stranded DNA intermediate
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Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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