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Invitrogen
Creatine Kinase BB Polyclonal Antibody
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FIGURE: 1 / 6
Creatine Kinase BB Antibody (PA5-27509) in ICC/IF
Creatine Kinase BB Polyclonal Antibody detects Creatine kinase (brain) protein at cytoplasm by immunofluorescent analysis. Sample: 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Creatine kinase (brain) stained by Creatine Kinase BB Polyclonal Antibody (Product # PA5-27509) diluted at 1:1,000. Blue: Fluoroshield with DAPI .
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-27509
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat,
Zebrafish
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human Creatine kinase (brain). The exact sequence is proprietary.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
2.05 mg/mL
Amount
205 µg
Purification
Protein A
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Recommended positive controls: 293T, H1299, HeLaS3, HepG2, NIH-3T3.
Predicted reactivity: Mouse (95%), Rat (96%), Xenopus laevis (85%), Pig (98%), Rabbit (96%), Chicken (89%), Rhesus Monkey (99%), Bovine (97%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
The protein encoded by this gene is a cytoplasmic enzyme involved in energy homeostasis. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in brain as well as in other tissues, and as a heterodimer with a similar muscle isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family. A pseudogene of this gene has been characterized.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: B-CK; brain creatine kinase; CKB; creatine kinase B chain; creatine kinase brain; creatine kinase brain-type; creatine kinase, brain; creatine kinase-B; creatine phosphokinase B-type; creatine phosphokinase M-type; epididymis luminal protein 211; epididymis secretory protein Li 29; unnamed protein product
Gene Aliases: B-CK; BCK; Ck-3; Ck3; CKBB; Ckbr; CPK-B; HEL-211; HEL-S-29
UniProt ID: (Human) P12277, (Rat) P07335, (Mouse) Q04447
Entrez Gene ID: (Human) 1152, (Rat) 24264, (Mouse) 12709
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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