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Invitrogen

AMPK alpha-1,2 Polyclonal Antibody

Advanced Verification
1 Published Figure
3 References
View all (20) AMPK alpha-1,2 antibodies

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AMPK alpha-1,2 Polyclonal Antibody

Product Details

PA5-36045

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:1,000
View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200
-

Immunoprecipitation (IP)

-
View 1 publication 1 publication
Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to amino acids 150-200 of human AMPK alpha1
3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Amount

100 µg

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2553341

Product Specific Information

This antibody detects endogenous protein at a molecular weight of 63 kDa.

Purity is >95% by SDS-PAGE.

Target Information

The protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: 5'-AMP-activated protein kinase, catalytic alpha-1 chain; 5'-AMP-activated protein kinase, catalytic alpha-2 chain; AAPK2; ACACA kinase; acetyl-CoA carboxylase kinase; AMP -activate kinase alpha 1 subunit; AMP-activated protein kinase; AMP-activated protein kinase, alpha 1 catalytic subunit; AMP-activated protein kinase, catalytic, alpha-1; AMPK alpha 2; AMPK alpha-1 chain; AMPK alpha-2 chain; AMPK alpha-2 variant 2; AMPK alpha-2 variant 3; AMPK subunit alpha-1; AMPK subunit alpha-2; AMPK, alpha, 1; AMPK-alpha-1 chain; AMPK-alpha-2 chain; HMGCR kinase; hydroxymethylglutaryl-CoA reductase kinase; pAMPK; protein kinase; protein kinase, AMP-activated, alpha 1 catalytic subunit; protein kinase, AMP-activated, alpha 2 catalytic subunit; tau-protein kinase PRKAA1; unnamed protein product

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Gene Aliases: 2310008I11Rik; A830082D05; AMPK; AMPK alpha 1; AMPK2; AMPKa1; AMPKa2; AMPKalpha1; AMPKalpha2; C130083N04Rik; PRKAA

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UniProt ID: (Human) Q13131, (Human) P54646, (Rat) P54645, (Mouse) Q5EG47, (Rat) Q09137, (Mouse) Q8BRK8

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Entrez Gene ID: (Human) 5562, (Human) 5563, (Rat) 65248, (Mouse) 105787, (Rat) 78975, (Mouse) 108079

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Function(s)
chromatin binding protein kinase activity protein serine/threonine kinase activity AMP-activated protein kinase activity cAMP-dependent protein kinase activity protein binding ATP binding macromolecular complex binding [hydroxymethylglutaryl-CoA reductase (NADPH)] kinase activity tau protein binding tau-protein kinase activity protein serine kinase activity histone H2BS36 kinase activity protein serine/threonine/tyrosine kinase activity eukaryotic elongation factor-2 kinase activator activity kinase binding protein binding, bridging
Process(es)
chromatin remodeling protein phosphorylation lipid biosynthetic process regulation of gene expression positive regulation of autophagy negative regulation of gene expression response to activity response to muscle activity regulation of macroautophagy peptidyl-serine phosphorylation regulation of lipid metabolic process response to caffeine cellular response to nutrient levels negative regulation of TOR signaling adiponectin-activated signaling pathway cellular response to oxidative stress cellular response to glucose starvation glucose homeostasis regulation of circadian rhythm negative regulation of apoptotic process positive regulation of glycolytic process fatty acid homeostasis regulation of stress granule assembly regulation of microtubule cytoskeleton organization cellular response to calcium ion cellular response to glucose stimulus cellular response to prostaglandin E stimulus cellular response to xenobiotic stimulus energy homeostasis positive regulation of cellular protein localization negative regulation of hepatocyte apoptotic process negative regulation of TORC1 signaling lipid droplet disassembly protein localization to lipid particle cytoplasmic translation phosphatidylethanolamine biosynthetic process phosphatidylcholine biosynthetic process cellular response to starvation positive regulation of catabolic process cellular response to amino acid starvation TORC1 signaling negative regulation of translational initiation positive regulation of translational initiation protein localization to lysosome protein K6-linked ubiquitination positive regulation of release of cytochrome c from mitochondria hepatocyte apoptotic process positive regulation of TORC1 signaling signal transduction positive regulation of macroautophagy positive regulation of T cell mediated immune response to tumor cell glucose metabolic process positive regulation of cell proliferation response to xenobiotic stimulus response to UV cold acclimation response to gamma radiation positive regulation of gene expression negative regulation of translation fatty acid oxidation regulation of peptidyl-serine phosphorylation cellular response to stress response to hydrogen peroxide regulation of vascular permeability response to estrogen positive regulation of gluconeogenesis positive regulation of transcription, DNA-templated positive regulation of fatty acid oxidation positive regulation of glucose import negative regulation of insulin receptor signaling pathway positive regulation of skeletal muscle tissue development positive regulation of T cell activation negative regulation of lipid catabolic process regulation of vesicle-mediated transport motor behavior CAMKK-AMPK signaling cascade neuron cellular homeostasis cellular response to hydrogen peroxide cellular response to ethanol cellular response to hypoxia protein localization to membrane protein localization to plasma membrane negative regulation of ferroptosis positive regulation of transcription from mitochondrial promoter positive regulation of establishment of protein localization to mitochondrion positive regulation of adipose tissue development autophagosome assembly negative regulation of T cell mediated immune response to tumor cell autophagy lysosome organization SCF-dependent proteasomal ubiquitin-dependent protein catabolic process protein destabilization proteasome-mediated ubiquitin-dependent protein catabolic process protein stabilization negative regulation of T cell activation protein-containing complex assembly negative regulation of protein localization to nucleus response to hypoxia positive regulation of cholesterol biosynthetic process negative regulation of glucosylceramide biosynthetic process
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