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Invitrogen
IFN alpha Polyclonal Antibody
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FIGURE: 1 / 2
IFN alpha Antibody (PA5-47984) in Neu
Neutralization of IFN alpha in LCRT cotton rat fibrosarcoma cell line. Samples were incubated in IFN alpha polyclonal antibody (Product # PA5-47984). Recombinant Cotton Rat IFN‚α (Cys109Tyr) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the LCRT cotton rat fibrosarcoma cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Inhibition of EMCV activity elicited by Recombinant Cotton Rat IFN‚α (Cys109Tyr) (0.2 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti... View More
Product Details
PA5-47984
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Rat
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived recombinant cotton rat IFN-alpha Cys24-Lys190 (Cys109Tyr)
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Lyophilized
Concentration
0.2 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
In direct ELISAs and Western blots, less than 2% cross-reactivity with recombinant human IFN-beta and recombinant cotton rat IFN-gamma is observed.
Reconstitute at 0.2 mg/mL in sterile PBS.
Endoxin level is <0.10 EU per 1 µg of the antibody by the LAL method.
Target Information
IFN alpha (Interferon alpha) is a Type I interferon belonging to a family of structurally related, species-specific proteins exclusive to vertebrates. The interaction of IFN-alpha with its receptor component result in the activation of a number of signaling pathways which are mainly involved in innate immune response against viral infection. In humans, the gene for IFN alpha is located on on chromosome 9. The type I interferons consist of 14 different alpha isoforms (subtypes with slightly different specificities), and single beta, omega, epsilon and kappa isoforms. Homologous molecules are found in many species, including rats and mice (and most mammals), and have been identified in birds, reptiles and fish species. IFN alpha is produced primarily by plasmacytoid dendritic cells, is a potent component of the anti-viral innate immune response and modulates adaptive immunity. Diseases associated with the dysfunction of IFN alpha include Hepatitis C and Hepatitis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ifa; IFN alpha 1/13; IFN alpha 1b; IFN α; IFN-ALPHA-2; IFN-ALPHAA; IFNA; IFNA2A; IFNA2B; IFNA2C; IFNAalphaIFN-alpha-1/13; IFNalpha; IFNα; Interferon alpha 13 (IFNA13); interferon alpha family, gene 2; Interferon alpha leukocyte (IFL); Interferon alpha-1; Interferon alpha-1/13 (IFN-alpha-1/13); Interferon alpha-D (IFN alphaD); Interferon alpha1 (IFNA1); LEIF A; MGC138207; MGC138505; MGC138507
Gene Aliases: IFN-alpha1; Ifna1
UniProt ID: (Rat) P05011
Entrez Gene ID: (Rat) 298210, (Rat) 298213
adaptive immune response
T cell activation involved in immune response
natural killer cell activation involved in immune response
humoral immune response
cytokine-mediated signaling pathway
B cell differentiation
positive regulation of peptidyl-serine phosphorylation of STAT protein
B cell proliferation
response to exogenous dsRNA
innate immune response
regulation of MHC class I biosynthetic process
defense response to virus
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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