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Invitrogen
N-WASP Polyclonal Antibody
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FIGURE: 1 / 13
N-WASP Antibody (PA5-52198) in ICC/IF
Immunofluorescence analysis of N-WASP was performed using 70% confluent log phase MCF7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 5 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with N-WASP Polyclonal Antibody (Product # PA5-52198) at 20 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conjugate (Prod... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-52198
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein corresponding to Human N-WASP. Recombinant protein control fragment (Product #RP-88621).
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.2 mg/mL
Amount
20 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 40% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: DHQVPTTAGN KAALLDQIRE GAQLKKVEQN SRPVSCSGRD ALLDQIRQGI QLKSVADGQE STPPTPAPTS GIVGALMEVM QKRS
Highest antigen sequence identity to the following orthologs: Mouse - 95%, Rat - 95%.
Target Information
The Wiskott-Aldrich syndrome (WAS) family of proteins share similar domain structure, and are involved in transduction of signals from receptors on the cell surface to the actin cytoskeleton. The presence of a number of different motifs suggests that they are regulated by a number of different stimuli, and interact with multiple proteins. Recent studies have demonstrated that these proteins, directly or indirectly, associate with the small GTPase, Cdc42, known to regulate formation of actin filaments, and the cytoskeletal organizing complex, Arp2/3. The WASL gene product is a homolog of WAS protein, however, unlike the latter, it is ubiquitously expressed and shows highest expression in neural tissues. It has been shown to bind Cdc42 directly, and induce formation of long actin microspikes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: DKFZp779G0847; MGC48327; n wasp; neural Wiskott-Aldrich syndrome protein; unnamed protein product; Wiskott-Aldrich syndrome like; Wiskott-Aldrich syndrome-like
Gene Aliases: 2900021I12Rik; 3110031I02Rik; N-WASP; NWASP; TRS4; WASPB
UniProt ID: (Human) O00401, (Mouse) Q91YD9
Entrez Gene ID: (Human) 8976, (Rat) 682507, (Mouse) 73178
membrane budding
actin filament organization
actin polymerization or depolymerization
vesicle organization
actin cytoskeleton organization
actin filament polymerization
vesicle transport along actin filament
positive regulation of transcription from RNA polymerase II promoter
positive regulation of filopodium assembly
dendritic spine morphogenesis
protein-containing complex assembly
formin-nucleated actin cable assembly
regulation of postsynapse organization
negative regulation of membrane tubulation
positive regulation of clathrin-mediated endocytosis
negative regulation of lymphocyte migration
response to bacterium
membrane invagination
protein complex localization
regulation of protein localization
positive regulation of chemotaxis
spindle localization
regulation of cell projection assembly
membrane tubulation
postsynaptic actin cytoskeleton organization
postsynapse organization
actin nucleation
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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