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Invitrogen
PRMT4 Polyclonal Antibody
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FIGURE: 1 / 3
PRMT4 Antibody (PA5-78913) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis of PRMT4 in Hela cells using PRMT4 Polyclonal Antibody (Product # PA5-78913). Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and incubated with the primary antibody at 5 µg/mL. DyLight 488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter set... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-78913
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human PRMT4 (267-284aa HLAPFTDEQLYMEQFTKA).
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Lyophilized
Concentration
500 µg/mL once reconstituted
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human 293T whole cell, human Hela whole cell, human MCF-7 whole cell, human HepG2 whole cell, rat testis tissue, rat C6 whole cell.
Target Information
Protein arginine N-methyltransferases, such as CARM1, catalyze the transfer of a methyl group from S-adenosyl-L-methionine to the side chain nitrogens of arginine residues within proteins to form methylated arginine derivatives and S-adenosyl-L-homocysteine. Protein arginine methylation has been implicated in DNA packaging, transcription regulation, signal transduction, mRNA stability, metabolism of nascent pre-RNA, and transcriptional activation. Tissue specificity: Overexpressed in prostate adenocarcinomas and high-grade prostatic intraepithelial neoplasia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: protein arginine N-methyltransferase 4
Gene Aliases: PRMT4
UniProt ID: (Human) Q86X55, (Rat) Q4AE70
Entrez Gene ID: (Human) 10498, (Rat) 363026
transcription regulatory region sequence-specific DNA binding
transcription coactivator activity
protein binding
beta-catenin binding
protein methyltransferase activity
protein-arginine N-methyltransferase activity
protein-arginine omega-N asymmetric methyltransferase activity
histone methyltransferase activity (H3-R17 specific)
histone methyltransferase activity
histone methyltransferase activity (H3-R2 specific)
DNA-binding transcription factor binding
histone H3R26 methyltransferase activity
regulation of growth plate cartilage chondrocyte proliferation
chromatin remodeling
regulation of transcription, DNA-templated
protein methylation
positive regulation of cell proliferation
gene expression
intracellular steroid hormone receptor signaling pathway
intracellular estrogen receptor signaling pathway
regulation of intracellular estrogen receptor signaling pathway
positive regulation of I-kappaB kinase/NF-kappaB signaling
positive regulation of fat cell differentiation
positive regulation of transcription from RNA polymerase I promoter
positive regulation of transcription from RNA polymerase II promoter
response to cAMP
endochondral bone morphogenesis
protein localization to chromatin
replication fork reversal
positive regulation of epithelial cell apoptotic process
negative regulation of dendrite development
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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