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Invitrogen
PRMT4 Polyclonal Antibody
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FIGURE: 1 / 3
PRMT4 Antibody (PA5-78913) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis of PRMT4 in Hela cells using PRMT4 Polyclonal Antibody (Product # PA5-78913). Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and incubated with the primary antibody at 5 µg/mL. DyLight 488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter set... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-78913
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human PRMT4 (267-284aa HLAPFTDEQLYMEQFTKA).
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Lyophilized
Concentration
500 µg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human 293T whole cell, human Hela whole cell, human MCF-7 whole cell, human HepG2 whole cell, rat testis tissue, rat C6 whole cell.
Target Information
Protein arginine N-methyltransferases, such as CARM1, catalyze the transfer of a methyl group from S-adenosyl-L-methionine to the side chain nitrogens of arginine residues within proteins to form methylated arginine derivatives and S-adenosyl-L-homocysteine. Protein arginine methylation has been implicated in DNA packaging, transcription regulation, signal transduction, mRNA stability, metabolism of nascent pre-RNA, and transcriptional activation. Tissue specificity: Overexpressed in prostate adenocarcinomas and high-grade prostatic intraepithelial neoplasia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Coactivator-associated arginine methyltransferase 1; coactivator-associated arginine methyltransferase 1 variant 5; Histone-arginine methyltransferase CARM1; Protein arginine N-methyltransferase 4
Gene Aliases: CARM1; PRMT4
UniProt ID: (Rat) Q4AE70
Entrez Gene ID: (Rat) 363026
transcription coactivator activity
protein binding
protein methyltransferase activity
histone-arginine N-methyltransferase activity
protein-arginine N-methyltransferase activity
ligand-dependent nuclear receptor transcription coactivator activity
protein-arginine omega-N asymmetric methyltransferase activity
histone methyltransferase activity (H3-R17 specific)
histone methyltransferase activity
protein homodimerization activity
transcription regulatory region DNA binding
lysine-acetylated histone binding
protein modifying enzyme
regulation of growth plate cartilage chondrocyte proliferation
transcription, DNA-templated
regulation of transcription, DNA-templated
mRNA processing
protein methylation
aging
positive regulation of cell proliferation
RNA splicing
pathogenesis
histone methylation
peptidyl-arginine methylation, to asymmetrical-dimethyl arginine
intracellular steroid hormone receptor signaling pathway
intracellular estrogen receptor signaling pathway
negative regulation of protein binding
regulation of intracellular estrogen receptor signaling pathway
histone arginine methylation
histone H3-R2 methylation
histone H3-R17 methylation
positive regulation of fat cell differentiation
response to cAMP
endochondral bone morphogenesis
regulation of mRNA binding
negative regulation of dendrite development
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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