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Invitrogen
HIF1AN Polyclonal Antibody
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FIGURE: 1 / 3
HIF1AN Antibody (PA5-81195) in ICC/IF
Immunofluorescence staining of HIF1N in HeLa cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with HIF1AN Polyclonal Antibody (Product # PA5-81195, 1:1,000) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to nucleus.
Product Details
PA5-81195
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to the C-terminus of the Human HIF1AN
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A, Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).
This antibody has specificity for Human HIF1AN.
Target Information
Hydroxylates HIF-1 alpha at 'Asn-803' in the C-terminal transactivation domain (CAD). Functions as an oxygen sensor and, under normoxic conditions, the hydroxylation prevents interaction of HIF-1 with transcriptional coactivators including Cbp/p300-interacting transactivator. Involved in transcriptional repression through interaction with HIF1A, VHL and histone deacetylases. Hydroxylates specific Asn residues within ankyrin repeat domains (ARD) of NFKB1, NFKBIA, NOTCH1, ASB4, PPP1R12A and several other ARD-containing proteins. Also hydroxylates Asp and His residues within ARDs of ANK1 and TNKS2, respectively. Negatively regulates NOTCH1 activity, accelerating myogenic differentiation. Positively regulates ASB4 activity, promoting vascular differentiation. [UniProt]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: co-repressor; DKFZp762F1811; Factor inhibiting HIF-1; factor inhibiting HIF1; FIH 1; FIH-1; FLJ20615; FLJ22027; hypoxia inducible factor 1 alpha subunit inhibitor; Hypoxia-inducible factor 1-alpha inhibitor; Hypoxia-inducible factor asparagine hydroxylase; peptide-aspartate beta-dioxygenase; unnamed protein product
Gene Aliases: FIH1; HIF1AN
UniProt ID: (Human) Q9NWT6
Entrez Gene ID: (Human) 55662
transcription corepressor activity
Notch binding
protein binding
ferrous iron binding
zinc ion binding
oxidoreductase activity
oxygen sensor activity
carboxylic acid binding
peptidyl-histidine dioxygenase activity
peptidyl-asparagine 3-dioxygenase activity
protein homodimerization activity
metal ion binding
NF-kappaB binding
dioxygenase activity
RNA polymerase II sequence-specific DNA binding transcription factor binding
peptidyl-aspartic acid 3-dioxygenase activity
ankyrin repeat binding
negative regulation of transcription from RNA polymerase II promoter
regulation of signal transduction
regulation of vascular endothelial growth factor receptor signaling pathway
positive regulation of myoblast differentiation
negative regulation of Notch signaling pathway
regulation of developmental process
positive regulation of vasculogenesis
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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