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Invitrogen

WNT1 Polyclonal Antibody

1 Published Figure
3 References
View all (14) WNT1 antibodies

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Product Details

PA5-85217

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:3,000
View 3 publications 3 publications
Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human Wnt1. The exact sequence is proprietary.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.82 mg/mL

Amount

82 µg

Purification

Antigen affinity chromatography

Storage buffer

0.1M tris glycine, pH 7, with 10% glycerol

Contains

0.01% thimerosal

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2792361

Product Specific Information

Keep as concentrated solution.

Predicted reactivity: Mouse (100%), Rat (99%), Zebrafish (80%), Bovine (99%).

Positive Control: WNT1 transfected 293T lysate.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Wnt proteins constitute a family of secreted glyocoproteins that activate signal transduction pathways to mediate tissue homeostasis, cell fate, cell proliferation, and self-renewal. The canonical signaling pathway is initiated upon Wnt binding to a Frizzled family receptor and an LRP5/LRP6 coreceptor, which leads to activation of the beta-catenin cascade. Non-canonical Wnt signals are activated by binding to a Frizzled family receptor and ROR2 coreceptor. Extensive research has shown that deregulation of these pathways or its components can lead to cancer. Human Wnt-1 is encoded as a 370 amino acid precursor that, upon secretion, is cleaved into a 343 amino acid (42 kDa) mature form. At the amino acid level, mature human Wnt-1 is approximately 99% identical to mature mouse Wnt-1. Depending on the tissue, Wnt-1 can activate the canonical and non-canonical signaling pathways. Studies have demonstrated its involvement in a variety of functions, including embryonic development and carcinogenesis. Wnt-1 signaling can also induce the phosphorylation and subsequent degradation of c-Myb.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: proto-oncogene Int-1; proto-oncogene Int-1 homolog; proto-oncogene protein Wnt-1; unnamed protein product; wingless-related MMTV integration site 1; Wingless-type MMTV integration site 1, homolog; wingless-type MMTV integration site family member 1; wingless-type MMTV integration site family, member 1; wingless-type MMTV integration site family, member 1 (oncogene INT1)

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Gene Aliases: BMND16; Int-1; INT1; OI15; sw; swaying; Wnt-1

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UniProt ID: (Human) P04628, (Mouse) P04426

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Entrez Gene ID: (Human) 7471, (Rat) 24881, (Mouse) 22408

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Function(s)
receptor binding frizzled binding cytokine activity morphogen activity protein domain specific binding receptor agonist activity protein binding
Process(es)
embryonic axis specification cell-cell signaling positive regulation of cell proliferation response to wounding positive regulation of lamellipodium assembly negative regulation of cell-substrate adhesion Wnt signaling pathway cerebellum development central nervous system morphogenesis cerebellum formation neurogenesis negative regulation of cell-cell adhesion neuron differentiation negative regulation of BMP signaling pathway midbrain development cell proliferation in midbrain astrocyte-dopaminergic neuron signaling signal transduction in response to DNA damage negative regulation of apoptotic process positive regulation of insulin-like growth factor receptor signaling pathway cell fate commitment negative regulation of cell differentiation negative regulation of fat cell differentiation positive regulation of Notch signaling pathway positive regulation of transcription, DNA-templated positive regulation of fibroblast proliferation Spemann organizer formation canonical Wnt signaling pathway bone development positive regulation of dermatome development dopaminergic neuron differentiation negative regulation of oxidative stress-induced neuron intrinsic apoptotic signaling pathway midbrain dopaminergic neuron differentiation embryonic brain development negative regulation of cellular senescence branching involved in ureteric bud morphogenesis positive regulation of protein phosphorylation myoblast fusion myotube differentiation spinal cord association neuron differentiation diencephalon development forebrain anterior/posterior pattern specification midbrain-hindbrain boundary maturation during brain development metencephalon development negative regulation of transforming growth factor beta receptor signaling pathway midbrain-hindbrain boundary development organ regeneration T cell differentiation in thymus inner ear morphogenesis positive regulation of transcription from RNA polymerase II promoter neuron fate commitment neuron fate determination hepatocyte differentiation cellular response to peptide hormone stimulus hematopoietic stem cell proliferation negative regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process regulation of transcription, DNA-templated signal transduction organ morphogenesis BMP signaling pathway
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