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Invitrogen
HIF-2 alpha Polyclonal Antibody
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FIGURE: 1 / 2
HIF-2 alpha Antibody (PA5-85520) in WB
HIF-2 alpha Polyclonal Antibody detects HIF2 alpha protein by western blot analysis. Un-treated (-) and treated (+, 1% O2 treatment for 24hr) HepG2 whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HIF-2 alpha Polyclonal Antibody (Product # PA5-85520) diluted by 1:500.
Product Details
PA5-85520
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human HIF2 alpha. The exact sequence is proprietary.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Keep as concentrated solution.
Predicted reactivity: Mouse (80%), Pig (83%).
Positive Control: HepG2 (1% O2 treatment for 24hr).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
HIF-2 alpha (Epas1) is a transcription factor involved in the induction of genes regulated by oxygen. HIF-2 alpha contains a basic-helix-loop-helix domain protein dimerization domain as well as a domain found in proteins in signal transduction pathways which respond to oxygen levels. It also regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of the lungs. Mutations in this gene are associated with erythrocytosis familial type 4.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Basic-helix-loop-helix-PAS protein MOP2; bHLHe73; Class E basic helix-loop-helix protein 73; Endothelial PAS domain-containing protein 1; EPAS-1; HIF-1-alpha-like factor; HIF-1alpha-like factor; HIF-2-alpha; HIF2-alpha; HLF; hypoxia-inducible factor 2 alpha; Hypoxia-inducible factor 2-alpha; Member of PAS protein 2; PAS domain-containing protein 2; unnamed protein product
Gene Aliases: BHLHE73; ECYT4; EPAS1; HIF2A; HLF; MOP2; PASD2
UniProt ID: (Human) Q99814
Entrez Gene ID: (Human) 2034
RNA polymerase II regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
core promoter proximal region sequence-specific DNA binding
transcription coactivator binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
transcription factor activity, sequence-specific DNA binding
protein binding
sequence-specific DNA binding
protein heterodimerization activity
protein dimerization activity
RNA polymerase II sequence-specific DNA binding transcription factor binding
angiogenesis
response to hypoxia
embryonic placenta development
blood vessel remodeling
regulation of heart rate
epithelial cell maturation
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
response to oxidative stress
mitochondrion organization
signal transduction
visual perception
gene expression
hemopoiesis
cell differentiation
erythrocyte differentiation
lung development
oxygen homeostasis
norepinephrine metabolic process
mRNA transcription from RNA polymerase II promoter
surfactant homeostasis
positive regulation of transcription from RNA polymerase II promoter
myoblast fate commitment
multicellular organismal iron ion homeostasis
cellular response to hypoxia
positive regulation of cold-induced thermogenesis
regulation of protein neddylation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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