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          • Primary Antibodies ›
          • FARSA Antibodies

          Invitrogen

          FARSA Polyclonal Antibody

          View all (10) FARSA antibodies

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          Cite FARSA Polyclonal Antibody

          Additional Information:
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          • Antibody Testing Data (1)
          FARSA Antibody in Western Blot (WB)
          Group 53 Created with Sketch.
          FARSA Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          FIGURE: 1 / 1

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          FARSA Antibody (PA5-90609) in WB

          Western blot analysis of FARSA in extracts of various cell lines. Samples were incubated with FARSA Polyclonal antibody (Product # PA5-90609) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          FARSA Antibody in Western Blot (WB)

          Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

          FARSA Polyclonal Antibody

          Product Details

          PA5-90609

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:2,000
          -

          ELISA (ELISA)

          1 µg/mL
          -
          Product Specifications

          Species Reactivity

          Human, Mouse

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Recombinant fusion protein containing a sequence corresponding to amino acids 210-486 of human FARSA (NP_0044521)
          3D Epitope / Immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1.36 mg/mL

          Amount

          136 µg

          Purification

          Affinity Chromatography

          Storage buffer

          PBS, pH 7.3, with 50% glycerol

          Contains

          0.01% thimerosal

          Storage conditions

          -20°C, Avoid Freeze/Thaw Cycles

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2806143

          Product Specific Information

          Immunogen sequence: YNFLAHGVLP DSGHLHPLLK VRSQFRQIFL EMGFTEMPTD NFIESSFWNF DALFQPQQHP ARDQHDTFFL RDPAEALQLP MDYVQRVKRT HSQGGYGSQG YKYNWKLDEA RKNLLRTHTT SASARALYRL AQKKPFTPVK YFSIDRVFRN ETLDATHLAE FHQIEGVVAD HGLTLGHLMG VLREFFTKLG ITQLRFKPAY NPYTEPSMEV FSYHQGLKKW VEVGNSGVFR PEMLLPMGLP ENVSVIAWGL SLERPTMIKY GINNIRELVG HKVNLQM; Positive Samples: HeLa, Mouse liver; Cellular Location: Cytoplasm

          Target Information

          Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. FARS2 is a phenylalanine-tRNA synthetase (PheRS) localized to the mitochondrion which consists of a single polypeptide chain, unlike the (alpha-beta)2 structure of the prokaryotic and eukaryotic cytoplasmic forms of PheRS. Structure analysis and catalytic properties indicate mitochondrial PheRSs may constitute a class of PheRS distinct from the enzymes found in prokaryotes and in the eukaryotic cytoplasm. Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. FARS1 encodes a mitochondrially-located phenylalanine-tRNA synthetase (PheRS) which consists of a single polypeptide chain unlike the (alpha-beta)2 structure of the prokaryotic and eukaryotic cytoplasmic forms of PheRS. Structure analysis and catalytic properties indicate mitochondrial PheRSs may constitute a class of PheRS distinct from the enzymes found in prokaryotes and the eukaryotic cytoplasm.

          ⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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          Cite this product

          Bioinformatics

          Protein Aliases: aminoacyl-tRNA synthetase; FARSLA; phenylalanine tRNA ligase 1, alpha, cytoplasmic; phenylalanine--tRNA ligase alpha chain; phenylalanine-tRNA synthetase alpha-subunit; phenylalanine-tRNA synthetase-like, alpha subunit; phenylalanyl-tRNA synthetase alpha chain; phenylalanyl-tRNA synthetase alpha subunit; phenylalanyl-tRNA synthetase-like, alpha subunit; pheRS; unnamed protein product

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          Gene Aliases: 0610012A19Rik; CML33; FARSL; FARSLA; FRSA; PheHA; pheRS; RILDBC2

          View more View less

          UniProt ID: (Human) Q9Y285, (Mouse) Q8C0C7

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          Entrez Gene ID: (Human) 2193, (Mouse) 66590

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          Function(s)
          tRNA binding nucleotide binding magnesium ion binding RNA binding aminoacyl-tRNA ligase activity phenylalanine-tRNA ligase activity protein binding ATP binding
          Process(es)
          phenylalanyl-tRNA aminoacylation tRNA aminoacylation protein heterotetramerization
          It has to be done as per old AB suggested Products section.

          Disclaimer

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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

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          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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