These Invitrogen Power Blotter Pre-cut Membranes and Filters are designed for the transfer of proteins from two mini-size or one midi-size gel using the Power Blotter System. The pre-cut membranes and filters include 20 sheets of polyvinylidene difluoride (PVDF) and 80 sheets of absorbent blotting paper (0.85 mm thickness). Power Blotter Pre-cut Membranes and Filters combined with Power Blotter 1-Step Transfer Buffer allow for high-efficiency semi-dry transfer of proteins in less than 10 minutes.
Features of PVDF membranes: • High-quality—PVDF transfer membranes manufactured especially for protein transfer and western blot applications • High-binding affinity—provides excellent protein binding (240 µg/cm2), blocks easily, and is compatible with most organic solvents, acids, and mild bases • Convenient—pre-cut PVDF sheets are ready to use for transfer and blotting experiments from two standard precast and homemade mini-gels or one midi-size gel (8 cm x 13 cm); no need to cut your own membranes, minimizing damage and contamination • Validated—membrane and filter when combined provide the thickness and buffer capacity needed for optimal performance in the Power Blotter System
The PVDF membrane (0.45 µm pore size, low fluorescence) has higher binding capacity than nitrocellulose. The membrane is compatible with commonly used detection methods such as staining, immunodetection, and fluorescence. The proteins bind to the membrane through hydrophobic interactions. This membrane has lower background levels and increased sensitivity for fluorescent probing than regular PVDF or nitrocellulose membranes.