Invitrogen Power Blotter 1-Step Transfer Buffer is designed for rapid semi-dry transfer of proteins from polyacrylamide gels (SDS-PAGE) to nitrocellulose or PVDF membranes using the Power Blotter System. Transfer is compatible with commonly used detection methods such as staining, chemiluminescence, or fluorescence.
Features of Power Blotter 1-Step Transfer Buffer: • Fast—high ionic strength formulation allows for 5- to 12-minute protein transfer when used with compatible fast semi-dry blotting systems • Optimized—designed for seamless and reliable performance with the Power Blotter System • Compatible—effective with other semi-dry transfer devices equipped with a suitable high-current power supply • Economical—no need for special or costly consumables that are required by other fast semi-dry transfer devices
When used with the Power Blotter System, this buffer provides effective gel-to-membrane transfer of proteins in 5 to 12 minutes with efficiency that is similar to conventional western blotting techniques. Fast blotting methods require a high-current power supply, such as the Power Blotter System, and an optimized high ionic strength transfer buffer, such as Power Blotter 1-Step Transfer Buffer.
By increasing the current, excellent transfer efficiency can be achieved in much shorter time compared to conventional methods. Amperage is held at a constant rate based on the surface area of the transfer stack(s) (~22–23 mA/cm2) and voltage is limited to 25 V.
Power Blotter 1-Step Transfer Buffer is also compatible with other protein semi-dry transfer devices, when they are paired with a suitable high-current power supply. Such devices provide constant high current (1.3 to 5.0 amps) to rapidly transfer proteins via the high ionic strength conditions supplied by the transfer buffer.