Invitrogen™

Power Blotter Pre-cut Membranes and Filters, nitrocellulose, regular size

Catalog number:  PB7320

 Related applications:

Protein Electrophoresis & Western Blotting

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Description

These Invitrogen Power Blotter Pre-cut Membranes and Filters are designed for transfer of proteins from two mini-size or one midi-size gel using the Power Blotter System. The pre-cut membranes and filters include 20 sheets of nitrocellulose and 80 sheets of absorbent blotting paper (0.85 mm thickness). Power Blotter Pre-cut Membranes and Filters combined with Power Blotter 1-Step Transfer Buffer allow for high-efficiency semi-dry transfer of proteins in less than 10 minutes.

Features of nitrocellulose membranes:
High-quality—pure 100% nitrocellulose membranes with high surface area and excellent uniformity
High-binding affinity—provides excellent protein binding (209 µg/cm2), blocks easily, and produces very low background in chemiluminescent and fluorescent western blotting
Convenient—pre-cut nitrocellulose sheets are ready to use for transfer and blotting experiments from two standard precast or homemade mini gels or one midi gel (8 cm x 13 cm); no need to cut your own membranes, minimizing damage and contamination
Validated—membrane and filter when combined provide the thickness and buffer capacity needed for optimal performance in the Power Blotter System

The nitrocellulose membrane (0.2 µM pore size) is composed of 100% pure nitrocellulose to provide high-quality transfers. Nitrocellulose is a popular binding matrix for western blotting because of its high affinity for proteins and compatibility with a variety of detection methods (e.g., chemiluminescence, chromogenic, fluorescence). The pre-cut membrane/filter paper sandwiches fit two mini-size or one midi-size gel.

Learn more about the Power Blotter System ›

Related products
Power Blotter Pre-cut Membranes and Filters, nitrocellulose, mini
Power Blotter Pre-cut Membranes and Filters, PVDF, mini
Power Blotter Pre-cut Membranes and Filters, PVDF, regular size

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Dimensions: 13cm x 8.5cm
Membrane: Nitrocellulose Membrane
Pore Size: 0.45 µm
Product Size: 20 stacks
Shipping Condition: Room Temperature

Contents & storage

20 regular-size transfer stacks (13 cm x 8.3 cm) composed of 20 sheets of nitrocellulose and 80 sheets of filter paper (0.85 mm thickness)

Documents

Manuals & protocols