Invitrogen™

Power Blotter Pre-cut Membranes and Filters, PVDF, regular size

Catalog number:  PB9320

 Related applications:

Protein Electrophoresis & Western Blotting

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Description

These Invitrogen Power Blotter Pre-cut Membranes and Filters are designed for the transfer of proteins from two mini-size or one midi-size gel using the Power Blotter System. The pre-cut membranes and filters include 20 sheets of polyvinylidene difluoride (PVDF) and 80 sheets of absorbent blotting paper (0.85 mm thickness). Power Blotter Pre-cut Membranes and Filters combined with Power Blotter 1-Step Transfer Buffer allow for high-efficiency semi-dry transfer of proteins in less than 10 minutes.

Features of PVDF membranes:
High-quality—PVDF transfer membranes manufactured especially for protein transfer and western blot applications
High-binding affinity—provides excellent protein binding (240 µg/cm2), blocks easily, and is compatible with most organic solvents, acids, and mild bases
Convenient—pre-cut PVDF sheets are ready to use for transfer and blotting experiments from two standard precast and homemade mini-gels or one midi-size gel (8 cm x 13 cm); no need to cut your own membranes, minimizing damage and contamination
Validated—membrane and filter when combined provide the thickness and buffer capacity needed for optimal performance in the Power Blotter System

The PVDF membrane (0.45 µm pore size, low fluorescence) has higher binding capacity than nitrocellulose. The membrane is compatible with commonly used detection methods such as staining, immunodetection, and fluorescence. The proteins bind to the membrane through hydrophobic interactions. This membrane has lower background levels and increased sensitivity for fluorescent probing than regular PVDF or nitrocellulose membranes.

Learn more about the Power Blotter System ›

Related products
Power Blotter Pre-cut Membranes and Filters, PVDF, mini
Power Blotter Pre-cut Membranes and Filters, nitrocellulose, mini
Power Blotter Pre-cut Membranes and Filters, nitrocellulose, regular size

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Dimensions: 13cm x 8.5cm
Membrane: PVDF Membrane
Pore Size: 0.45 µm
Product Size: 20 stacks
Shipping Condition: Room Temperature

Contents & storage

20 regular-size transfer stacks (13 cm x 8.3 cm) composed of 20 sheets of PVDF and 80 sheets of filter paper (0.85 mm thickness)

Documents

Manuals & protocols