These Invitrogen Power Blotter Pre-cut Membranes and Filters are designed for transfer of proteins from mini-size gels using the Power Blotter System. The pre-cut membranes and filters include 20 sheets of nitrocellulose and 80 sheets of absorbent blotting paper (0.85 mm thickness). Power Blotter Pre-cut Membranes and Filters combined with Power Blotter 1-Step Transfer Buffer allow for high-efficiency semi-dry transfer of proteins in less than 10 minutes.
Features of nitrocellulose membranes: • High-quality—pure 100% nitrocellulose membranes with high surface area and excellent uniformity • High-binding affinity—provides excellent protein binding (209 µg/cm2), blocks easily, and produces very low background in chemiluminescent and fluorescent western blotting • Convenient—pre-cut nitrocellulose sheets are ready to use for transfer and blotting experiments from standard precast or homemade mini gels (8 cm x 8 cm); no need to cut your own membranes, minimizing damage and contamination • Validated—membrane and filter when combined provide the thickness and buffer capacity needed for optimal performance in the Power Blotter System
The nitrocellulose membrane (0.45 µM pore size) is composed of 100% pure nitrocellulose to provide high-quality transfers. Nitrocellulose is a popular binding matrix for western blotting because of its high affinity for proteins and compatibility with a variety of detection methods (e.g., chemiluminescence, chromogenic, fluorescence). These pre-cut membrane/filter paper sandwiches fit mini sized gels.