Thermo Scientific™

ROX Solution, 50 µM

Catalog number: R1371
Thermo Scientific™

ROX Solution, 50 µM

Catalog number: R1371
Catalog Number
R1371
Unit Size
1 mL
Price (USD)
99.50
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
R13711 mL
99.50
Your Price:
***
Product Overview
Figures
Videos
Documents
FAQ
Citations & References
Additional Information
Recommendations
Recommendations
Thermo Scientific ROX dye is added to the qPCR reactions to serve as an internal reference for normalization of the fluorescent signal when using real-time instruments that can detect ROX. ROX allows for the correction of well-to-well variation appearing due to pipetting inaccuracies and fluorescence fluctuations. It can be added to an entire 2X master mix tube or to an individual reaction mixture (for recommended amounts of ROX Solution for a specific real-time instrument refer to the qPCR manual).

ROX does not participate in PCR and does not interfere with the specificity or sensitivity of qPCR assays.

ROX Solution contains ROX dye as a 50 µM aqueous solution at pH 7.6. The excitation maximum and emission maximum of ROX are at 580 nm and 621 nm, respectively.

Specifications

Concentration
50 µM
Detection Method
Fluorescence
Fidelity (vs. Taq)
2 X
Format
Tube
GC-Rich PCR Performance
Low
Label Type
Fluorescent Dye
PCR Method
qPCR, RT-PCR
Volume
1 mL
Label or Dye
ROX
Product Line
ROX
Product Type
qPCR Reagents

Contents & Storage

Contains:
• 1 mL ROX Solution, 50 µMStore at -20°C.

Figures