Tarjetas iBind™ Flex
Tarjetas iBind™ Flex
Invitrogen™

Tarjetas iBind™ Flex

Las tarjetas iBind se utilizan con el dispositivo iBind Flex Western, que es un dispositivo de procesamiento de Western automatizadoMás información
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Número de catálogoTipoCantidad
SLF2010X4Tarjeta40 tarjetas (10 x 4)
SLF2010Tarjeta10 tarjetas
Número de catálogo SLF2010X4
Precio (MXN)
-
Tipo:
Tarjeta
Cantidad:
40 tarjetas (10 x 4)
Las tarjetas iBind se utilizan con el dispositivo iBind Flex Western, que es un dispositivo de procesamiento de Western automatizado que realiza todos los pasos del flujo de trabajo de Western, desde el bloqueo y el lavado hasta la incubación de anticuerpos. Cada solución pasa de los pocillos del dispositivo iBind Flex a la tarjeta iBind Flex mediante el flujo lateral secuencial (SLF); luego, las soluciones se transfieren hacia la zona de la pila de la tarjeta. La matriz de fibra de vidrio de la tarjeta permite el flujo uniforme y homogéneo de las soluciones hacia la membrana, lo que aumenta la interacción entre el antígeno y el anticuerpo.

Obtenga más información sobre los sistemas iBind Western ›

Las tarjetas iBind Flex, junto con el dispositivo iBind Flex Western y el kit solución iBind Flex y el kit de solución de detección fluorescente (FD) iBind Flex, aportan automatización y sencillez al protocolo Western. Cada tarjeta iBind Flex puede procesar hasta un blot midi, dos blots mini o seis blots en tiras de corte vertical.

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones
Cantidad40 tarjetas (10 x 4)
Condiciones de envíoTemperatura ambiente
CapacidadUna transferencia Midi, seis transferencias de tiras cortadas verticalmente, dos transferencias Mini
Método de detecciónCromogénico, quimioluminiscente, fluorescente
Para utilizar con (aplicación)Inmunotransferencia (Western Blotting)
Para utilizar con (equipo)Dispositivo iBind Flex Western
Membrane CompatibilityNitrocelulosa, PVDF
Línea de productosiBind Flex
Running Time∼2,5 h
Capacidad de procesamientoUna transferencia Midi, seis transferencias de tiras cortadas verticalmente, dos transferencias Mini por tarjeta
TipoTarjeta
Unit Size10 cards,40 cards
Contenido y almacenamiento
Almacenar a temperatura ambiente.

Preguntas frecuentes

Can I use conjugated primary antibodies with the iBind Flex Western Device?

Yes. When using a conjugated primary antibody and no secondary antibody, add the diluted primary antibody to well 1 and replace diluted secondary antibody (well 3) with 1X iBind Flex/iBind Flex FD Solution. Alternatively, for faster time to results, add the diluted primary antibody to well 1, leave wells 2 and 3 empty, and add 1X iBind Flex/ iBind Flex FD Solution to well 4. This procedure will save time (~60 min) but may result in higher background.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

What procedure should I follow when stripping and reprobing my blot processed using the iBind Western Device or iBind Flex Western Device?

To strip and reprobe your blot processed using the iBind Western Device or iBind Flex Western Device, first strip the blot outside the system using your preferred protocol. After the stripping protocol, wash the blot in distilled water for 5 min. Reblocking is unnecessary as the iBind Solution will perform this step during reprobing. Finally, reprobe with new antibodies using the iBind Western Device or iBind Flex Western Device, starting with the membrane preparation step.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Can I multiplex antibodies using the iBind Western Device or iBind Flex Western Device?

Yes, for each midi blot, mini blot, or vertically cut strip, prepare appropriate primary antibodies together in a single tube of iBind FD/ iBind Flex FD Solution, Prepare appropriate secondary antibodies together in a different tube of iBind FD/ iBind Flex FD Solution. Do not mix primary and secondary antibodies prior to loading in the device. All primary and secondary antibodies should be diluted according to the recommendations outlined in the user manual or quick reference card. When detecting several targets of the same species, the same concentration of secondary antibody can be used as when detecting one target. There is no need to increase the concentration for each additional target. Load multiplexed antibodies into the device as normal.
Caution: Consider cross-reactivity of secondary antibodies when multiplexing (e.g., rabbit anti-goat IgG and goat anti-mouse IgG are likely to cross-react).

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Can I use the iBind Western Device or iBind Flex Western Device with Alexa Fluor Plus conjugated secondary antibodies?

Yes, both the iBind Western Device and iBind Flex Western Device work well with Alexa Fluor Plus conjugated secondary antibodies.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

With the iBind Western Device and iBind Flex Western Device, how do I know if I am rolling the membrane firmly enough?

When firmly rolling, the iBind/iBind Flex Card will slightly dip in the membrane area, causing the top and bottom of the card to lift. See the iBind/iBind Flex user manual for additional details.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

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