pMT/V5-His A, B, & C Drosophila Expression Vectors

Catalog number: V412020

Invitrogen™  Related applications: Protein Expression

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Description

The DES®-Inducible Kit provides the expression vector pMT/V5-His and a choice of Blasticidin (pCoBlast) or hygromycin (pCoHygro) selection. This vector uses the Drosophila metallothionein gene promoter that is induced in S2 cells upon addition of copper sulfate or cadmium chloride to the culture medium.

The pMT/V5-His vector offers the following features:

• Small size (3.5 kb) to improve DNA yields and increase subcloning efficiency
• C-terminal V5 epitope tag for rapid detection with Invitrogen's Anti-V5 Antibody
• C-terminal 6xHis tag for simple purification of recombinant fusion proteins using nickel-chelating resin

To facilitate cloning, a set of three vectors-A, B, and C-is provided. Each vector has the multiple cloning site in a different reading frame relative to the coding sequence of the C-terminal tag.
For Research Use Only. Not for use in diagnostic procedures.

Figures

Specifications

Cloning Method: Restriction Enzyme ⁄ MCS
Product Size: 20 µg
Promoter: MT
Protein Tag Position (to your gene): C-terminal
Protein Tag or Fusion: His Tag (6x), V5 Epitope Tag
Vector Type: pMT

Contents & storage

• 20 µg each of pMT/V5-His A, B, and C (0.5 µg/µl in 10 mM Tris-HCl, 1 mM EDTA, pH 8.0 in a volume of 40 µl)
• 20 µg pMT/V5-His/lacZ (0.5 µg/µl in 10 mM Tris-HCl, 1 mM EDTA, pH 8.0 in a volume of 40 µl)

Store at -30°C to -10°C.

Documents

Manuals & protocols