Kit de inicio iBind™ Flex Western, detección por fluorescencia

El kit de inicio iBind Flex Western para la detección de fluorescencia incluye un dispositivo iBind Flex Western, tarjetas iBindMás información

Número de catálogo	Cantidad
SLF2002S	1 Kit

Número de catálogo SLF2002S

Precio (USD)

Cantidad:

1 Kit

El kit de inicio iBind Flex Western para la detección de fluorescencia incluye un dispositivo iBind Flex Western, tarjetas iBind Flex y un kit de solución de detección fluorescente (FD) iBind Flex El dispositivo iBind Flex Western es un pequeño dispositivo de sobremesa que automatiza el procesamiento de Western blot (inmunodetección). Aplica presión mecánica a una tarjeta iBind Flex para generar flujo lateral secuencial (SLF) de soluciones de inmunodetección para los pasos de bloqueo, de unión de anticuerpos y de lavado. La matriz de fibra de vidrio de la tarjeta iBind Flex permite un flujo homogéneo y consistente de las soluciones sobre la membrana, aumentando la interacción antígeno y el anticuerpo.

El dispositivo iBind Flex Western permite que todas las soluciones se preparen y se carguen en el dispositivo al inicio del procedimiento; todos los pasos siguientes se realizan automáticamente a través de la acción SLF, no requiere electricidad ni baterías. Los blots están listos para los pasos finales de detección en aproximadamente dos horas y media.

Más información sobre los sistemas iBind Western›

El dispositivo iBind Flex Western incluye tres insertos de pocillos intercambiables por el usuario para procesar hasta un midiblot, dos miniblots, o 6 blots de tiras cortadas verticalmente cuando se utilizan con tarjetas iBind Flex, y con el kit de soluciones iBind Flex o el kit de soluciones de detección fluorescente (FD) iBind Flex para procesar un miniblot.

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones

Cantidad1 Kit

Condiciones de envíoTemperatura ambiente

CapacidadUna transferencia Midi, seis transferencias de tiras cortadas verticalmente, dos transferencias Mini

Método de detecciónFluorescente

Para utilizar con (aplicación)Inmunotransferencia (Western Blotting)

Membrane CompatibilityNitrocelulosa, PVDF

Línea de productosiBind

Running Time∼2,5 h

Capacidad de procesamientoUna transferencia Midi, seis transferencias de tiras cortadas verticalmente, dos transferencias Mini

TipoKit de inicio

Unit Size1 kit

Contenido y almacenamiento

Un dispositivo iBind Flex Western, 10 tarjetas iBind (almacenar a temperatura ambiente), un kit de solución de detección por fluorescencia (FD) iBind Flex (almacenar de 2 a 8 °C)

Preguntas frecuentes

Can I use conjugated primary antibodies with the iBind Flex Western Device?

Yes. When using a conjugated primary antibody and no secondary antibody, add the diluted primary antibody to well 1 and replace diluted secondary antibody (well 3) with 1X iBind Flex/iBind Flex FD Solution. Alternatively, for faster time to results, add the diluted primary antibody to well 1, leave wells 2 and 3 empty, and add 1X iBind Flex/ iBind Flex FD Solution to well 4. This procedure will save time (~60 min) but may result in higher background.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

What procedure should I follow when stripping and reprobing my blot processed using the iBind Western Device or iBind Flex Western Device?

To strip and reprobe your blot processed using the iBind Western Device or iBind Flex Western Device, first strip the blot outside the system using your preferred protocol. After the stripping protocol, wash the blot in distilled water for 5 min. Reblocking is unnecessary as the iBind Solution will perform this step during reprobing. Finally, reprobe with new antibodies using the iBind Western Device or iBind Flex Western Device, starting with the membrane preparation step.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Can I multiplex antibodies using the iBind Western Device or iBind Flex Western Device?

Yes, for each midi blot, mini blot, or vertically cut strip, prepare appropriate primary antibodies together in a single tube of iBind FD/ iBind Flex FD Solution, Prepare appropriate secondary antibodies together in a different tube of iBind FD/ iBind Flex FD Solution. Do not mix primary and secondary antibodies prior to loading in the device. All primary and secondary antibodies should be diluted according to the recommendations outlined in the user manual or quick reference card. When detecting several targets of the same species, the same concentration of secondary antibody can be used as when detecting one target. There is no need to increase the concentration for each additional target. Load multiplexed antibodies into the device as normal.
Caution: Consider cross-reactivity of secondary antibodies when multiplexing (e.g., rabbit anti-goat IgG and goat anti-mouse IgG are likely to cross-react).

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Can I use the iBind Western Device or iBind Flex Western Device with Alexa Fluor Plus conjugated secondary antibodies?

Yes, both the iBind Western Device and iBind Flex Western Device work well with Alexa Fluor Plus conjugated secondary antibodies.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

With the iBind Western Device and iBind Flex Western Device, how do I know if I am rolling the membrane firmly enough?

When firmly rolling, the iBind/iBind Flex Card will slightly dip in the membrane area, causing the top and bottom of the card to lift. See the iBind/iBind Flex user manual for additional details.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.