PDPH (3-(2-pyridyldithio)propionyl hydrazide)

Zitierungen und Referenzen (3)

Thermo Scientific™

PDPH (3-(2-pyridyldithio)propionyl hydrazide)

Thermo Scientific Pierce PDPH ist ein kurzer Pyridylldithiol-und-Hydrazid-Crosslinker für die reversible Konjugation von Thiolen (Cystein) in Carbonyle (Aldehyd oder Ketone,Weitere Informationen

Katalognummer	Menge
22301	50 mg

Katalognummer 22301

Preis (EUR)

283,00

Menge:

50 mg

Preis (EUR)

283,00

Thermo Scientific Pierce PDPH ist ein kurzer Pyridylldithiol-und-Hydrazid-Crosslinker für die reversible Konjugation von Thiolen (Cystein) in Carbonyle (Aldehyd oder Ketone, wie sie durch Oxidation von Glykoprotein-Kohlenhydraten entstehen).

Merkmale von PDPH:

• Reaktive Gruppen: Pyridylldisulfid und Hydrazid
• Reaktiv gegen: Sulfhydrylgruppen und Carbonylgruppen (Aldehyd)
• Kurzer (9,2 A), Sulfhydryl-zu-Aldehyd-Crosslinker mit Disulfid-Bondspacerarm (Cleleer)
• Pyridylldithiol-Gruppe führt über Disulfid-Bindung zu einer Bindung an Sulfhydrylen, welche mit DTT, TCEP oder anderen Reduktionsmitteln gespalten werden können
• Hydrazid-Gruppe konjugiert zu oxidierten Zuckern von Glykoproteinen und Kohlenhydraten
• Natrium-Meta-Periodat, um Glykosylierung (z. B. Sialsäure) zu reaktiven Aldehydgruppen zu oxidieren
• EDC verwenden, um Primäramin der Hydrazid-Gruppe an Carboxylgruppen zu konjugieren

Produktreferenzen:
Crosslinker Anwendungsleitfaden -- Suchen Sie nach aktuellen Literaturreferenzen für dieses Produkt

Nur für Forschungszwecke. Nicht zur Verwendung bei diagnostischen Verfahren.

Specifications

ZellpermeabilitätJa

BeschreibungPDPH

FormPulver

MarkierungsmethodeChemische Markierung

Molekulargewicht229,32

PEGyliertNein

ProduktliniePierce

Menge50 mg

Reaktiver TeilHydrazid, Pyridyldisulfid

VersandbedingungUmgebungstemperatur

LöslichkeitDMF, DMSO

Länge des Spacer-Arms9,2 Å

WasserlöslichNein

Chemische ReaktivitätKohlenhydrat-Sulfhydryl

CleavableVon Thiols

Crosslinker-TypHeterobifunktional

FormatStandard

ProdukttypCrosslinker

AbstandshalterKurz (<10 Å)

Unit SizeEach

Inhalt und Lagerung

Nach Erhalt getrocknet bei 4 °C lagern.

Häufig gestellte Fragen (FAQ)

Can you provide the shelf-life for PDPH (3-(2-pyridyldithio)propionyl hydrazide)?

PDPH (3-(2-pyridyldithio)propionyl hydrazide) is covered under our general 1-year warranty and is guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended. Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf) for more details.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

We are using EDC to crosslink a protein onto carboxyl-modified beads. We would like to add in a linker molecule to extend the radius of beads to increase the surface area so more proteins can be immobilized. Is this possible?

To provide a linker between the beads and the target protein, you would need a molecule with a primary amine (to conjugate to the carboxyl group on the beads with EDC) on one end and another reactive group on the other (to conjugate to the protein). For instance, sulfo-SMCC (Cat. No. A39268) could be used in conjunction with PDPH (Cat. No. 22301):
The method is outlined below:
1. Conjugate the target protein to the NHS ester (amine-reactive) group of Sulfo-SMCC. This will produce a maleimide-activated target protein via its primary amines (lysines and N-terminus).
2. Remove the excess sulfo-SMCC by desalting or dialysis.
3. React the maleimide-activated target protein to PDPH via sulfhydryls. (Lysines and N-terminus now are connected to hydrazide (amine) group with an extended linker)
4. Remove excess PDPH by desalting or dialysis.
5. React the hydrazide-activated target protein with carboxyl beads in the presence of EDC.
6. Use appropriate buffer to rinse off excess target protein from the carboxyl beads.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Zitierungen und Referenzen (3)

Zitierungen und Referenzen

Abstract

Systemic administration of a nerve growth factor conjugate reverses age-related cognitive dysfunction and prevents cholinergic neuron atrophy.

Authors:Bäckman C, Rose GM, Hoffer BJ, Henry MA, Bartus RT, Friden P, Granholm AC

Journal:J Neurosci

PubMed ID:8757256

'Intraventricular administration of nerve growth factor (NGF) in rats has been shown to reduce age-related atrophy of central cholinergic neurons and the accompanying memory impairment. Intraventricular administration of NGF is necessary because NGF will not cross the blood-brain barrier (BBB). Here we have used a novel carrier system, consisting of ... More

Conjugation of folate via gelonin carbohydrate residues retains ribosomal-inactivating properties of the toxin and permits targeting to folate receptor positive cells.

Authors:Atkinson SF, Bettinger T, Seymour LW, Behr JP, Ward CM

Journal:J Biol Chem

PubMed ID:11359781

Conjugation of folate to proteins permits receptor-mediated endocytosis via the folate receptor (FR) and delivery of the conjugate into the cytoplasm of cells. Since many cancers up-regulate the FR it has enabled the targeting of toxins to tumor cells resulting in specific cell death. However, current conjugation methods rely on ... More

Site-specific modifications of light chain glycosylated antilymphoma (LL2) and anti-carcinoembryonic antigen (hImmu-14-N) antibody divalent f1agments.

Authors:Govindan SV, Goldenberg DM, Griffiths GL, Leung SO, Losman MJ, Hansen HJ

Journal:Cancer Res

PubMed ID:7493334

Site-specific introduction of metal-chelating groups into F(ab')2 fragments of an antilymphoma antibody (LL2) possessing a natural Asn-linked light chain carbohydrate and an anti-carcinoembryonic antigen antibody (hImmu-14-N) grafted with a light chain carbohydrate site is described. For this purpose, four yttrium- (and indium)-chelating agents were used, containing a primary amino group ... More