Insulin-Transferrin-Selen (ITS-G) (100X)
Try new Gibco Insulin-Transferrin-Selenium Select supplements, an animal-origin free formulation. Available in ITS-G & ITS-X. Learn more >
Insulin-Transferrin-Selen (ITS-G) (100X)
Insulin-Transferrin-Selen (ITS-G) (100X)
Insulin-Transferrin-Selen (ITS-G) (100X)
Zitierungen und Referenzen (4)
Gibco™

Insulin-Transferrin-Selen (ITS-G) (100X)

Gibco™ Insulin-Transferrin-Selen (ITS-G) wird als Basalmedium-Supplement verwendet, um die Menge an fötalem Kälberserum (FBS) zu reduzieren, das zur Zellkultivierung benötigtWeitere Informationen
Have Questions?
KatalognummerMenge
4140004510 ml
Katalognummer 41400045
Preis (EUR)
67,75
Each
Zum Warenkorb hinzufügen
Menge:
10 ml
Preis (EUR)
67,75
Each
Zum Warenkorb hinzufügen
Gibco™ Insulin-Transferrin-Selen (ITS-G) wird als Basalmedium-Supplement verwendet, um die Menge an fötalem Kälberserum (FBS) zu reduzieren, das zur Zellkultivierung benötigt wird. Die ursprünglich mit RPMI 1640 und Minimum Essential Medium (MEM) verwendete ITS-G-Lösung wird mittlerweile routinemäßig mit anderen Basalmedien zur Unterstützung verschiedenster Zelltypen mit 2 bis 4 % FBS eingesetzt.

Insulin fördert die Aufnahme von Glukose und Aminosäure, die Lipogenese, den intrazellulären Transport und die Synthese von Proteinen und Nukleinsäuren. Transferrin ist ein Eisenträger, der auch zur Senkung toxischer Konzentrationen von Sauerstoffradikalen und Wasserstoffperoxid beitragen kann. Selen ist als Natriumselenit ein Kofaktor für Glutathionperoxidase und andere Proteine und wird als Antioxidans in Medien verwendet.

Die komplette Formulierung ist erhältlich.

cGMP-konformes Herstellungs- und Qualitätssystem
Gibco™ ITS-G wird in einer cGMP-konformen Anlage in Grand Island, New York, hergestellt. Die Einrichtung ist von der US-Arzneimittelbehörde FDA als Hersteller von Medizinprodukten zugelassen und nach ISO 13485-zertifiziert.
Nur für Forschungszwecke. Nicht zur Verwendung bei diagnostischen Verfahren.
Specifications
ZelltypSäugetierzellen
Konzentration100 X
Menge10 ml
Haltbarkeit18 Monate
VersandbedingungRaumtemperatur
FormFlüssig
ProdukttypTransferrin
SterilitätSteril gefiltert
Ohne AdditiveKein Phenolrot
Unit SizeEach
Inhalt und Lagerung
Lagerbedingungen: 2 bis 8 °C
Versandbedingungen: Haltbarkeit bei
Raumtemperatur: 18 Monate ab Herstellungsdatum

Häufig gestellte Fragen (FAQ)

What is the buffer composition of the various Insulin-Transferrin-Selenium (ITS) (100X) and Insulin-Transferrin-Selenium (ITS) Select (100X) supplements?

All ITS and ITS Select supplements are formulated in Earle's Balanced Salt Solution (EBSS).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the difference between Insulin-Transferrin-Selenium (ITS) (100X) and Insulin-Transferrin-Selenium (ITS) Select (100X) supplements?

ITS Select supplements are animal origin-free (AOF) versions of the ITS supplements offered by Thermo Fisher Scientific.

ITS Select supplements use recombinant versions of both proteins in the formulation: insulin and transferrin.

All other versions of ITS, those without the Select designation, utilize recombinant insulin and a human-derived holo transferrin.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What are the individual concentrations of insulin, transferrin, and selenium in the Insulin-Transferrin-Selenium (ITS) (100X) supplements?

All ITS and ITS Select supplements have the following concentrations of their components:

Insulin: 1.0 g/L
Transferrin: 0.55 g/L
Selenium: 0.00067 g/L

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can I store aliquots of Insulin-Transferrin-Selenium (ITS) (100X) supplements at -20 degrees C without compromising the integrity of the product?

We recommend storing all ITS and ITS Select supplements at 2-8 degrees C as there is a chance that the insulin will precipitate upon freezing and thawing.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Are all Insulin-Transferrin-Selenium (ITS) (100X) supplements offered by Thermo Fisher Scientific animal origin-free?

We offer both animal origin and animal origin-free versions of our ITS supplements. The animal origin-free product versions are referred to as ITS Select supplements.

Insulin-Transferrin-Selenium (ITS-G) (100X) (Cat. No. 41400045), Insulin-Transferrin-Selenium-Ethanolamine (ITS-X) (100X) (Cat. No. 51500056), and Insulin-Transferrin-Selenium-Sodium Pyruvate (ITS-A) (100X) (Cat. No. 51300044) are not animal origin-free.

Animal origin-free product versions are available for the ITS-G and ITS-X formulations: Insulin-Transferrin-Selenium (ITS-G) Select Supplement (100X) (Cat. No. A4000046501 and A4000046502) and Insulin-Transferrin-Selenium-Ethanolamine (ITS-X) Select Supplement (100X) (Cat. No. A4000046401 and Cat. No. A4000046402.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all Insulin-Transferrin-Selen (ITS-G) (100X) FAQs

Zitierungen und Referenzen (4)

Zitierungen und Referenzen
Abstract
A comparison between different human hepatocyte models reveals profound differences in net glucose production, lipid composition and metabolism in vitro.
Authors:Bonanini F,Singh M,Yang H,Kurek D,Harms AC,Mardinoglu A,Hankemeier T
Journal:Experimental cell research
PubMed ID:38499143
Protocol to generate human liver spheroids to study liver fibrosis induced by metabolic stress.
Authors:Kim HY,Lee W,Liu X,Jang H,Sakane S,Carvalho-Gontijo Weber R,Diggle K,Kerk SA,Metallo CM,Kisseleva T,Brenner DA
Journal:STAR protocols
PubMed ID:38833372
Currently, there is no effective treatment for obesity and alcohol-associated liver diseases, partially due to the lack of translational human models. Here, we present a protocol to generate 3D human liver spheroids that contain all the liver cell types and mimic “livers in a dish.” We describe strategies to induce ... More
Collective cell migration requires suppression of actomyosin at cell-cell contacts mediated by DDR1 and the cell polarity regulators Par3 and Par6.
Authors:Hidalgo-Carcedo C, Hooper S, Chaudhry SI, Williamson P, Harrington K, Leitinger B, Sahai E,
Journal:Nat Cell Biol
PubMed ID:21170030
Collective cell migration occurs in a range of contexts: cancer cells frequently invade in cohorts while retaining cell-cell junctions. Here we show that collective invasion by cancer cells depends on decreasing actomyosin contractility at sites of cell-cell contact. When actomyosin is not downregulated at cell-cell contacts, migrating cells lose cohesion. ... More
Human cardiomyocyte progenitor cells differentiate into functional mature cardiomyocytes: an in vitro model for studying human cardiac physiology and pathophysiology.
Authors:Smits AM, van Vliet P, Metz CH, Korfage T, Sluijter JP, Doevendans PA, Goumans MJ,
Journal:Nat Protoc
PubMed ID:19197267
To date, there is no suitable in vitro model to study human adult cardiac cell biology. Although embryonic stem cells are able to differentiate into cardiomyocytes in vitro, the efficiency of this process is very low. Other methods to differentiate progenitor cells into beating cardiomyocytes rely on coculturing with rat ... More